Sensitive and High-Throughput Isolation of Rare Cells from Peripheral Blood with Ensemble-Decision Aliquot Ranking

Enrichment of cells: An approach called ensemble‐decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood is described. The eDAR process has a recovery of over 93 % (number of runs n=9) with a zero false positive rate (n=8) and provides direct easy access to individual isolated...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2012-05, Vol.51 (19), p.4618-4622
Main Authors: Schiro, Perry G., Zhao, Mengxia, Kuo, Jason S., Koehler, Karen M., Sabath, Daniel E., Chiu, Daniel T.
Format: Article
Language:English
Subjects:
Antibodies - immunology
Antigens, Neoplasm - immunology
Antigens, Neoplasm - metabolism
Blood Cells - cytology
cancer
Cell Adhesion Molecules - immunology
Cell Adhesion Molecules - metabolism
cell capture
cells
Epithelial Cell Adhesion Molecule
Flow Cytometry
Fluorescent Dyes - chemistry
high-throughput screening
High-Throughput Screening Assays
Humans
Microscopy, Confocal
Neoplastic Cells, Circulating
Neoplastic Stem Cells - cytology
Neoplastic Stem Cells - metabolism
Receptor, ErbB-2 - immunology
Receptor, ErbB-2 - metabolism
single-cell analysis
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Summary:Enrichment of cells: An approach called ensemble‐decision aliquot ranking (eDAR) for isolating rare cells from peripheral blood is described. The eDAR process has a recovery of over 93 % (number of runs n=9) with a zero false positive rate (n=8) and provides direct easy access to individual isolated live cells for downstream single‐cell manipulation and analysis (CTCs=circulating tumor cells).
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.201108695