Transcription of DNA histone complexes by yeast RNA polymerase B

Transcription of denatured DNA complexed with histones ( total, H1 or H2A/H2B/H3/H4 ) by yeast RNA polymerase B is investigated. Binding of histones to DNA restricts its template activity by decreasing the formation of active, heparin-resistant, RNA polymerase initiation complexes. The elongation of...

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Bibliographic Details
Published in:Nucleic acids research 1978-06, Vol.5 (6), p.1907-1917
Main Authors: Karagyozov, L.K, Valkanov, M.A, Hadjiolov, A.A
Format: Article
Language:English
Subjects:
Animals
Deoxyribonucleoproteins - metabolism
DNA - metabolism
DNA-Directed RNA Polymerases - metabolism
Heparin - pharmacology
Histones - metabolism
Kinetics
Nucleoproteins - metabolism
Rats
RNA Polymerase II - metabolism
Saccharomyces cerevisiae
Saccharomyces cerevisiae - enzymology
Templates, Genetic
Transcription, Genetic - drug effects
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Summary:Transcription of denatured DNA complexed with histones ( total, H1 or H2A/H2B/H3/H4 ) by yeast RNA polymerase B is investigated. Binding of histones to DNA restricts its template activity by decreasing the formation of active, heparin-resistant, RNA polymerase initiation complexes. The elongation of pre-initiated RNA on denatured DNA, complexed with histones, is possible, although resulting in somewhat shorter RNA chains. It is suggested that RNA polymerase B can elongate on a DNA strand covered with histones.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/5.6.1907