The relationship between the activities of different pools of RNA polymerases I and II during PHA-stimulation of human lymphocytes

Following PHA-stimulation of lymphocytes in culture, it is known that nuclear RNA synthesis and the amount of extractable RNA polymerase activity rise in these cells. The relationship between these two phenomena has been examined. Using an in vitro assay system which discriminates between polymerase...

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Bibliographic Details
Published in:Nucleic acids research 1978-06, Vol.5 (6), p.2099-2112
Main Authors: Tillyer, Colin R., Butterworth, Peter H. W.
Format: Article
Language:English
Subjects:
Adult
Cell Nucleus - enzymology
Cells, Cultured
Cytoplasm - enzymology
Dactinomycin - pharmacology
DNA-Directed RNA Polymerases - metabolism
Humans
Kinetics
Lectins
Lymphocyte Activation
Lymphocytes - drug effects
Lymphocytes - enzymology
RNA Polymerase I - metabolism
RNA Polymerase II - metabolism
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Summary:Following PHA-stimulation of lymphocytes in culture, it is known that nuclear RNA synthesis and the amount of extractable RNA polymerase activity rise in these cells. The relationship between these two phenomena has been examined. Using an in vitro assay system which discriminates between polymerase activity which is “engaged” in nuclear RNA synthesis and a pool of “free” enzyme, the data suggest that the factors regulating the inter action between these two pools of enzyme activity are different for forms I and II RNA polymerases.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/5.6.2099