Non-polyadenylated 22 S ribonucleoprotein particle is insensitive to translational inhibitor RNA of cryptobiotic gastrulae of Artemia salina

A free cytoplasmic 22 S ribonucleoprotein particle exhibiting a major template activity in rabbit reticulocyte system has been identified in the crypto-biotic gastrulae of Artemia salina. This particle contains non-polyadenylated 9 S messenger UNA which codes primarily for a non-histone basic protei...

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Bibliographic Details
Published in:Nucleic acids research 1979-11, Vol.7 (5), p.1363-1374
Main Authors: Herdt, Erik De, Slegers, Herman, Piot, Etienne, Kondo, Masatoshi
Format: Article
Language:English
Subjects:
Animals
Artemia - metabolism
Artemia salina
Chromosomal Proteins, Non-Histone - biosynthesis
Kinetics
Nucleic Acid Hybridization
Nucleoproteins - metabolism
Poly A - metabolism
Poly U
Protein Biosynthesis
Ribonucleoproteins - isolation & purification
Ribonucleoproteins - metabolism
RNA, Messenger - isolation & purification
RNA, Messenger - metabolism
Templates, Genetic
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Summary:A free cytoplasmic 22 S ribonucleoprotein particle exhibiting a major template activity in rabbit reticulocyte system has been identified in the crypto-biotic gastrulae of Artemia salina. This particle contains non-polyadenylated 9 S messenger UNA which codes primarily for a non-histone basic protein with an apparent molecular weight of 26 000 daltons. We have previously demonstrated the presence of a translational inhibitor RNA which is apparently responsible for transforming polyadenylated messenger ribonucleoproteins into a repressed state in the cryptobiotic gastrulae (Siegers et al., FEBS Letters 80, 390–394, 1977). This inhibitor RNA was found to be completely ineffective on the template activity of non-polyadenylated 22 S messenger ribonucleoprotein, confirming the specificity of this regulatory RNA for polyadenylate sequences.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/7.5.1363