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Hexose Transporter Expression and Function in Mouse Small Intestine: Role of Diurnal Rhythm
Background Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice. Aim The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel. Meth...
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Published in: | Journal of gastrointestinal surgery 2009-04, Vol.13 (4), p.634-641 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Background
Expression and function of hexose transporters vary diurnally in rat small intestine; however, this subject remains unexplored in mice.
Aim
The aim of the study was to investigate the diurnal expression and function of hexose transporters SGLT1, GLUT2, and GLUT5 in mouse small bowel.
Methods
Twenty-four c57bl6 mice maintained in a 12-h light/dark room (6
am
–6
pm
) were sacrificed at 9
am
, 3
pm
, 9
pm
, and 3
am
(
n
= 6 each). In duodenal, jejunal, and ileal mucosa, total cellular mRNA and protein levels were quantitated by real-time PCR and semiquantitative Western blotting, respectively. The everted sleeve technique measured transporter-mediated glucose uptake at 9
am
and 9
pm
.
Results
mRNA expression of SGLT1, GLUT2, and GLUT5 varied diurnally in all three intestinal segments (
p
≤ 0.03). SGLT1, GLUT2, and GLUT5 protein levels varied diurnally in duodenum and jejunum (
p
70% of food intake occurred; glucose transport followed a similar pattern with increased uptake at 9
pm
.
Conclusion
Hexose transporter expression and function vary diurnally with nocturnal feeding patterns of mice. |
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ISSN: | 1091-255X 1873-4626 |
DOI: | 10.1007/s11605-008-0776-4 |