Transcription of single base oligonucleotides by ribonucleic acid-directed deoxyribonucleic acid polymerase

The synthesis of DNA products complementary to artificial templates by the enzyme RNA-directed DNA polymerase isolated from avian myeloblastosis virus has been studied. Of the single base polyribonucleotides, poly(rC), poly(rA), and poly(rI) were active while poly(rG) and poly(rU) were almost inacti...

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Bibliographic Details
Published in:Nucleic acids research 1976-01, Vol.3 (1), p.79-88
Main Authors: Falvey, Amy K., Weiss, Gary B., Krueger, Leslie J., Kantor, Judith A., Anderson, W. French
Format: Article
Language:English
Subjects:
Avian myeloblastosis virus
Avian Myeloblastosis Virus - enzymology
Kinetics
Molecular Weight
Oligodeoxyribonucleotides - metabolism
Oligonucleotides - metabolism
Oligoribonucleotides - metabolism
RNA-Directed DNA Polymerase - metabolism
Structure-Activity Relationship
Templates, Genetic
Transcription, Genetic
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Summary:The synthesis of DNA products complementary to artificial templates by the enzyme RNA-directed DNA polymerase isolated from avian myeloblastosis virus has been studied. Of the single base polyribonucleotides, poly(rC), poly(rA), and poly(rI) were active while poly(rG) and poly(rU) were almost inactive. The minimum length showing activity for an oligo(rC) template was 9; the minimum primer length of oligo(dG) was 3 or 4. In order to examine the fidelity of transcription, single base oligoribonucleotides of defined length were studied. Using (rC)13as template and (dG)8 as primer, the oligo(dG) product coelectrophoresed with the template. However, using (rA)20 as template and (dT)10 as primer, a large (10–16S) product was formed. Similarly, using oligo(rI) (2. 5S) as template and (dC)10 as primer, a large (>22S) product was formed. No significant activity was obtained with oligo(rU) templates. RNA-directed DNA polymerase transcribes the various oligonucleotides differently: slippage with oligo(rA) and oligo(rl), faithful transcription with oligo(rC), and poor transcription with oligo(rU).
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/3.1.79