Interpretation of the properties of chromatin extracts from mammalian nuclei

Chromatin from standard preparations of nuclei stabilized by magnesium ions at 0–4°C was degraded during the nuclear isolation, and newly-synthesized chromatin was degraded slightly more slowly than the ‘older’ chromatin. The significance of this observation, and its relation to the interpretation o...

Full description

Saved in:
Bibliographic Details
Published in:Nucleic acids research 1976-04, Vol.3 (4), p.1101-1110
Main Authors: Marshall, A.J., Burgoyne, L.A.
Format: Article
Language:English
Subjects:
Animals
Calcium - pharmacology
Cell Fractionation
Cell Nucleus - analysis
Cell Nucleus - metabolism
Chromatin - isolation & purification
Chromatin - metabolism
Endonucleases - metabolism
Enzyme Activation - drug effects
Liver - metabolism
Liver Regeneration
Magnesium - pharmacology
Micrococcus - enzymology
Molecular Weight
Rats
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Chromatin from standard preparations of nuclei stabilized by magnesium ions at 0–4°C was degraded during the nuclear isolation, and newly-synthesized chromatin was degraded slightly more slowly than the ‘older’ chromatin. The significance of this observation, and its relation to the interpretation of the properties of nucleoprotein extracts is discussed.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/3.4.1101