Effect of β,β-dimethylacrylshikonin on inhibition of human colorectal cancer cell growth in vitro and in vivo

In traditional Chinese medicine, shikonin and its derivatives, has been used in East Asia for several years for the prevention and treatment of several diseases, including cancer. We previously identified that β,β-dimethylacrylshikonin (DA) could inhibit hepatocellular carcinoma growth. In the prese...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular sciences 2012, Vol.13 (7), p.9184-9193
Main Authors: Fan, Yingying, Jin, Shaoju, He, Jun, Shao, Zhenjun, Yan, Jiao, Feng, Ting, Li, Hong
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
bcl-2-Associated X Protein - metabolism
bcl-X Protein - metabolism
BH3 Interacting Domain Death Agonist Protein - metabolism
Cell Line, Tumor
Cell Proliferation - drug effects
Colorectal Neoplasms - drug therapy
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
Dose-Response Relationship, Drug
Humans
Male
Mice
Mice, Nude
Naphthoquinones - pharmacology
Proto-Oncogene Proteins c-bcl-2 - metabolism
Xenograft Model Antitumor Assays
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In traditional Chinese medicine, shikonin and its derivatives, has been used in East Asia for several years for the prevention and treatment of several diseases, including cancer. We previously identified that β,β-dimethylacrylshikonin (DA) could inhibit hepatocellular carcinoma growth. In the present study, we investigated the inhibitory effects of DA on human colorectal cancer (CRC) cell line HCT-116 in vitro and in vivo. A viability assay showed that DA could inhibit tumor cell growth in a time- and dose-dependent manner. Flow cytometry showed that DA blocks the cell cycle at G(0)/G(1) phase. Western blotting results demonstrated that the induction of apoptosis by DA correlated with the induction of pro-apoptotic proteins Bax, and Bid, and a decrease in the expression of anti-apoptotic proteins Bcl-2 and Bcl-xl. Furthermore, treatment of HCT-116 bearing nude mice with DA significantly retarded the growth of xenografts. Consistent with the results in vitro, the DA-mediated suppression of HCT-116 xenografts correlated with Bax and Bcl-2. Taken together, these results suggest that DA could be a novel and promising approach to the treatment of CRC.
ISSN:1422-0067
1422-0067
DOI:10.3390/ijms13079184