Chick chorioallantoic membrane (CAM) assay as an in vivo model to study the effect of newly identified molecules on ovarian cancer invasion and metastasis

The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the...

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Bibliographic Details
Published in:International journal of molecular sciences 2012-08, Vol.13 (8), p.9959-9970
Main Authors: Lokman, Noor A, Elder, Alison S F, Ricciardelli, Carmela, Oehler, Martin K
Format: Article
Language:English
Subjects:
Animals
Antibodies, Neutralizing - pharmacology
Apoptosis
Biological Assay
Cell Adhesion
Cell Movement
Cell Proliferation
Chick Embryo
Chorioallantoic Membrane
Female
Flow Cytometry
Humans
Mice
Neoplasm Invasiveness
Neoplasm Metastasis
Ovarian cancer
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Staphylococcal Protein A - chemistry
Staphylococcal Protein A - immunology
Staphylococcal Protein A - metabolism
Tumor Cells, Cultured
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Summary:The majority of ovarian cancer patients present with advanced disease and despite aggressive treatment, prognosis remains poor. Significant improvement in ovarian cancer survival will require the development of more effective molecularly targeted therapeutics. Commonly, mouse models are used for the in vivo assessment of potential new therapeutic targets in ovarian cancer. However, animal models are costly and time consuming. Other models, such as the chick embryo chorioallantoic membrane (CAM) assay, are therefore an attractive alternative. CAM assays have been widely used to study angiogenesis and tumor invasion of colorectal, prostate and brain cancers. However, there have been limited studies that have used CAM assays to assess ovarian cancer invasion and metastasis. We have therefore developed a CAM assay protocol to monitor the metastatic properties of ovarian cancer cells (OVCAR-3, SKOV-3 and OV-90) and to study the effect of potential therapeutic molecules in vivo. The results from the CAM assay are consistent with cancer cell motility and invasion observed in in vitro assays. Our results demonstrate that the CAM assay is a robust and cost effective model to study ovarian cancer cell metastasis. It is therefore a very useful in vivo model for screening of potential novel therapeutics.
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms13089959