Determination of the sequence homology between the four RNA species of cucumber mosaic virus by hybridization analysis with complementary DNA

The method of Taylor etal., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 - 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary...

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Bibliographic Details
Published in:Nucleic acids research 1977-11, Vol.4 (11), p.3787-3802
Main Authors: Gould, A.R, Symons, R.H
Format: Article
Language:English
Subjects:
Base Sequence
Cucumber mosaic virus
DNA, Viral
Kinetics
Mosaic Viruses
Nucleic Acid Hybridization
plant health
Plant Viruses
RNA, Viral
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Summary:The method of Taylor etal., (11) has been used to transcribe complementary DNA probes from the four major RNA species of cucumber mosaic virus (RNAs 1 - 4 in order of decreasing molecular weight). Analysis of the kinetics of hybridization of these probes in homologous and heterologous complementary DNA-RNA hybridization reactions has shown that the sequence of the smallest RNA (RNA 4), which contains the coat protein gene, is present within RNA 3. RNAs 1 and 2 are unique RNA molecules while each has a region of approximately 300 nucleotides in common with RNA 4.Images
ISSN:0305-1048
1362-4962