An ADIOL-ERβ-CtBP Transrepression Pathway Negatively Regulates Microglia-Mediated Inflammation

• Published in: Cell 2011-05, Vol.145 (4), p.584-595
• Main Authors: Saijo, Kaoru, Collier, Jana G., Li, Andrew C., Katzenellenbogen, John A., Glass, Christopher K.
• Format: Article
• Language: English
• Subjects: 17-Hydroxysteroid Dehydrogenases - metabolism; agonists; Alcohol Oxidoreductases - metabolism; Androstenediol - metabolism; Animals; astrocytes; Astrocytes - metabolism; Cells, Cultured; central nervous system; DNA-Binding Proteins - metabolism; encephalitis; Encephalomyelitis, Autoimmune, Experimental - metabolism; Encephalomyelitis, Autoimmune, Experimental - prevention & control; estradiol; Estrogen Receptor beta - metabolism; estrogen receptors; genes; homeostasis; Humans; inflammation; Inflammation - metabolism; ligands; Mice; Mice, Inbred C57BL; Microglia - metabolism; Neurodegenerative Diseases - metabolism; Proto-Oncogene Proteins c-fos - metabolism; Signal Transduction; T-lymphocytes; Toll-like receptor 4
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2011.03.050

Microglia and astrocytes play essential roles in the maintenance of homeostasis within the central nervous system, but mechanisms that control the magnitude and duration of responses to infection and injury remain poorly understood. Here, we provide evidence that 5-androsten-3β,17β-diol (ADIOL) functions as a selective modulator of estrogen receptor (ER)β to suppress inflammatory responses of microglia and astrocytes. ADIOL and a subset of synthetic ERβ-specific ligands, but not 17β-estradiol, mediate recruitment of CtBP corepressor complexes to AP-1-dependent promoters, thereby repressing genes that amplify inflammatory responses and activate Th17 T cells. Reduction of ADIOL or ERβ expression results in exaggerated inflammatory responses to TLR4 agonists. Conversely, the administration of ADIOL or synthetic ERβ-specific ligands that promote CtBP recruitment prevents experimental autoimmune encephalomyelitis in an ERβ-dependent manner. These findings provide evidence for an ADIOL/ERβ/CtBP-transrepression pathway that regulates inflammatory responses in microglia and can be targeted by selective ERβ modulators. [Display omitted] [Display omitted] ► ERβ functions as an ADIOL receptor in the brain to repress inflammatory responses ► ADIOL production is regulated by inflammatory signaling ► ADIOL induces ERβ recruitment of CtBP corepressors to proinflammatory promoters ► Synthetic ADIOL-like ERβ modulators suppress disease in a mouse model of MS