The 1-Carbamoyl-2-oxo-4,5-dihydroxyimidazolidine Component of ROS-Induced DNA Damage in White Blood Cells

The 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine is a known base modification produced in vitro by oxidative stress. However, the presence of this modification in vivo has not been established. In this study the introduction of this base modification into dinucleoside monoph...

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Bibliographic Details
Published in:Radiation research 2010-07, Vol.174 (1), p.101-106
Main Authors: Iijima, Herbert, Patrzyc, Helen B., Budzinski, Edwin E., Dawidzik, Jean B., Freund, Harold G., Box, Harold C.
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid
DNA Damage
Humans
Imidazolidines - pharmacology
Leukocytes - drug effects
Leukocytes - metabolism
Reactive Oxygen Species - metabolism
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Space life sciences
Tandem Mass Spectrometry
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Summary:The 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine is a known base modification produced in vitro by oxidative stress. However, the presence of this modification in vivo has not been established. In this study the introduction of this base modification into dinucleoside monophosphates was accomplished using the Fenton reaction. Subsequently, the modification was produced in short isotopically labeled oligomers. Labeled tetramers bearing the lesion were used as internal standards for LC-MS/MS determinations of the base modification in the DNA of white blood cells from healthy donors. The background level of the 1-carbamoyl-2-oxo-4,5-dihydroxyimidazolidine modification of cytosine was found to be larger than the levels of the formamide and thymine glycol base modifications.
ISSN:0033-7587
1938-5404
DOI:10.1667/RR2087.1