microRNA-192, -194 and -215 are frequently downregulated in colorectal cancer

microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total...

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Bibliographic Details
Published in:Experimental and therapeutic medicine 2012-03, Vol.3 (3), p.560-566
Main Authors: CHIANG, YEUNPO, SONG, YONGXI, WANG, ZHENNING, LIU, ZHUANGKAI, GAO, PENG, LIANG, JIWANG, ZHU, JINLIANG, XING, CHENGZHONG, XU, HUIMIAN
Format: Article
Language:English
Subjects:
colorectal cancer
microRNA
miR-192
miR-194
miR-215
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Summary:microRNAs (miRNAs) are small, non-coding RNAs of endogenous origin. They have been increasingly shown to have aberrant expression in a number of tumor types. miR-192, -194 and -215 have not been comprehensively investigated using a large number of cases in colorectal cancer (CRC). We extracted total RNA from 107 CRC tissues and three CRC cell lines. Following polyadenylation and reverse transcription, the expression levels of miR-192, -194 and -215 were determined for evaluation of the association between expression levels and clinicopathological characteristics by a quantitative real-time polymerase chain reaction (real-time PCR) method. Finally, we studied the impact of miR-194 on cell proliferation in HCT-116 cells by MTT assay. miR-192, -194 and -215 were significantly downregulated in CRC tissues (all p
ISSN:1792-0981
1792-1015
DOI:10.3892/etm.2011.436