The role of molecular chaperonins in warm ischemia and reperfusion injury in the steatotic liver: a proteomic study

• Published in: BMC biochemistry 2012-09, Vol.13 (1), p.17-17
• Main Authors: Tiriveedhi, Venkataswarup, Conzen, Kendra D, Liaw-Conlin, Jane, Upadhya, Gundumi, Malone, James, Townsend, R Reid, Kerns, Robnet, Jia, Jianluo, Csontos, Krista, Ramachandran, Sabarinathan, Mohanakumar, Thallachallour, Anderson, Christopher D, Chapman, William C
• Format: Article
• Language: English
• Subjects: Abdomen; Animals; Chromatography, High Pressure Liquid; Databases, Protein; Disease susceptibility; Down-Regulation; Electrophoresis, Gel, Two-Dimensional; Endoplasmic Reticulum Stress; Fatty Liver - metabolism; Fatty Liver - pathology; Heat shock proteins; Ischemia; Laboratory animals; Liver; Liver diseases; Male; Mass Spectrometry; Medicine; Molecular chaperones; Molecular Chaperones - metabolism; Mortality; Obesity; Oxidative stress; Physiological aspects; Protein binding; Proteins; Proteome - metabolism; Proteomics; Rats; Rats, Zucker; Reperfusion injury; Reperfusion Injury - metabolism; Risk factors; Rodents; Surgery; Transplantation of organs, tissues, etc; Transplants & implants; Up-Regulation; Warm Ischemia; Writing
• ISSN: 1471-2091; 1471-2237; 1471-2091
• DOI: 10.1186/1471-2091-13-17

The molecular basis of the increased susceptibility of steatotic livers to warm ischemia/reperfusion (I/R) injury during transplantation remains undefined. Animal model for warm I/R injury was induced in obese Zucker rats. Lean Zucker rats provided controls. Two dimensional differential gel electrophoresis was performed with liver protein extracts. Protein features with significant abundance ratios (p