Polarized multispectral imaging in a rigid endoscope based on elastic light scattering spectroscopy

Elastic light scattering spectroscopy (LSS) is widely utilized to investigate cellular structures in cultured cells and various tissues. However, few imaging systems, especially endoscopic imaging systems, can implement LSS. It is the aim of this work to create a polarized multispectral imaging syst...

Full description

Saved in:
Bibliographic Details
Published in:Biomedical optics express 2012-09, Vol.3 (9), p.2087-2099
Main Authors: Qi, Ji, Barrière, Clement, Wood, Tobias C, Elson, Daniel S
Format: Article
Language:English
Subjects:
Endoscopes, Catheters and Micro-Optics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Elastic light scattering spectroscopy (LSS) is widely utilized to investigate cellular structures in cultured cells and various tissues. However, few imaging systems, especially endoscopic imaging systems, can implement LSS. It is the aim of this work to create a polarized multispectral imaging system based around a rigid endoscope to detect micrometer sized particles using LSS. The instrument first validated with different sized mono-disperse polystyrene microspheres, then an image is reconstructed based on LSS which shows the differentiation of different sized microspheres. Finally a preliminary experiment is conducted to demonstrate its capability to discriminate different types of cells.
ISSN:2156-7085
2156-7085
DOI:10.1364/boe.3.002087