impact of dehydration rate on the production and cellular location of reactive oxygen species in an aquatic moss

Background and AimsThe aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore...

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Bibliographic Details
Published in:Annals of botany 2012-10, Vol.110 (5), p.1007-1016
Main Authors: Cruz de Carvalho, Ricardo, Catalá, Myriam, Marques da Silva, Jorge, Branquinho, Cristina, Barreno, Eva
Format: Article
Language:English
Subjects:
Bryophytes
Bryopsida - cytology
Bryopsida - metabolism
Bryopsida - physiology
Cell Survival
cell viability
confocal laser scanning microscopy
Dehydration
Desiccation
Drying
Fontinalis antipyretica
hydrogen peroxide
Hydrogen Peroxide - analysis
Hydrogen Peroxide - metabolism
Microscopy, Confocal
Mosses
mosses and liverworts
Oral rehydration
Original
Oxidative stress
Oxidative Stress - physiology
Oxygen - metabolism
Oxygen consumption
Plant Leaves - cytology
Plant Leaves - metabolism
Plant Leaves - physiology
Plant physiology
Plants
Reactive oxygen species
Reactive Oxygen Species - metabolism
rehydration
Time Factors
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Summary:Background and AimsThe aquatic moss Fontinalis antipyretica requires a slow rate of dehydration to survive a desiccation event. The present work examined whether differences in the dehydration rate resulted in corresponding differences in the production of reactive oxygen species (ROS) and therefore in the amount of cell damage.MethodsIntracellular ROS production by the aquatic moss was assessed with confocal laser microscopy and the ROS-specific chemical probe 2,7-dichlorodihydrofluorescein diacetate. The production of hydrogen peroxide was also quantified and its cellular location was assessed.Key ResultsThe rehydration of slowly dried cells was associated with lower ROS production, thereby reducing the amount of cellular damage and increasing cell survival. A high oxygen consumption burst accompanied the initial stages of rehydration, perhaps due to the burst of ROS production.ConclusionsA slow dehydration rate may induce cell protection mechanisms that serve to limit ROS production and reduce the oxidative burst, decreasing the number of damaged and dead cells due upon rehydration.
ISSN:0305-7364
1095-8290
DOI:10.1093/aob/mcs180