MVA recombinants expressing the fusion and hemagglutinin genes of PPRV protects goats against virulent challenge

Peste des Petits Ruminants (PPR) is a highly contagious animal disease caused by the Peste des Petits Ruminants virus (PPRV) belonging to the genus morbillivirus and family Paramyxoviridae. The disease results in high morbidity and mortality in goats, sheep and in some small wild ruminants. The pres...

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Bibliographic Details
Published in:Indian journal of microbiology 2010-09, Vol.50 (3), p.266-274
Main Authors: Chandran, Dev, Reddy, Kolli Bhaktavatsala, Vijayan, Shahana Pallichera, Sugumar, Parthasarthy, Rani, Gudavalli Sudha, Kumar, Ponsekaran Santha, Rajendra, Lingala, Srinivasan, Villuppanoor Alwar
Format: Article
Language:English
Subjects:
Animal diseases
Animals
Biomedical and Life Sciences
Cloning
Embryos
Epidemics
Genes
Glycoproteins
Goats
Life Sciences
Medical Microbiology
Microbiology
Original
Original Article
Proteins
Vaccines
Virulence
Viruses
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Summary:Peste des Petits Ruminants (PPR) is a highly contagious animal disease caused by the Peste des Petits Ruminants virus (PPRV) belonging to the genus morbillivirus and family Paramyxoviridae. The disease results in high morbidity and mortality in goats, sheep and in some small wild ruminants. The presence of large number of small ruminants reared in endemic areas makes PPR a notorious disease threatening the livelihood of poor farmers. Conventional vaccination using a live, attenuated vaccine gives adequate protection but cannot be used in case of eradication of the disease due to difficulty in differentiation of infected animals from the vaccinated ones. In the present study, we constructed two recombinant viruses using attenuated Modified Vaccinia virus Ankara virus (MVA) namely MVA-F and MVA-H expressing the full length PPRV fusion (F) and hemagglutinin (H) glycoproteins, respectively. Goats were vaccinated intramuscularly with 105 plaque forming units (PFU) each of the recombinant viruses and a live attenuated vaccine (RAKSHA PPR) and challenged 4 months later with PPRV challenge virus (10 3 goat LD 50 ). All goats were completely protected from the clinical disease. This study gave an indication that mass vaccination of small ruminants with either of the above or both recombinant inexpensive virus vaccines could help in possible eradication of PPRV from endemic countries like India and subsequent seromonitoring of the disease for differentiation of infected animals from vaccinated ones.
ISSN:0046-8991
0973-7715
DOI:10.1007/s12088-010-0026-9