Protoplast formation and regeneration in Lactobacillus delbrueckii

Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa an...

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Published in:Indian journal of microbiology 2010-03, Vol.50 (1), p.97-100
Main Authors: Singhvi, Mamta, Joshi, Dipti, Gaikaiwari, Shalaka, Gokhale, Digambar V.
Format: Article
Language:English
Subjects:
Acids
Biomedical and Life Sciences
Enzymes
Genomes
Life Sciences
Medical Microbiology
Microbiology
Microorganisms
Original
Original Article
Sucrose
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description Method for production and regeneration of Lactobacillus delbrueckii protoplasts are described. The protoplasts were obtained by treatment with a mixture of lysozyme and mutanolysin in protoplast buffer at pH 6.5 with different osmotic stabilizers. The protoplasts were regenerated on deMan, Rogosa and Sharpe (MRS) with various osmotic stabilizers. Maximum protoplast formation was obtained in protoplast buffer with sucrose as an osmotic stabilizer using a combination of lysozyme (1 mg/ml) and mutanolysin (10 μg/ml). Maximum protoplast regeneration was obtained on MRS medium with sucrose (0.5 M) as an osmotic stabilizer. The regeneration medium was also applicable to other species of lactobacilli as well. This is, to our knowledge, the first report on protoplast formation and efficient regeneration in case of L. delbrueckii .
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subjects Acids
Biomedical and Life Sciences
Enzymes
Genomes
Life Sciences
Medical Microbiology
Microbiology
Microorganisms
Original
Original Article
Sucrose
title Protoplast formation and regeneration in Lactobacillus delbrueckii
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