Loading…

Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column

A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were id...

Full description

Saved in:
Bibliographic Details
Published in:Indian journal of clinical biochemistry 2000-07, Vol.15 (2), p.119-123
Main Authors: Basu, P S, Majhi, R, Chatterjee, S, Batabyal, S K
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by
cites cdi_FETCH-LOGICAL-c2799-2dcbc38dc630ae6cab5dd9711c93d3057bf5c7f3d603b6ca535ebc1bcc6fadcb3
container_end_page 123
container_issue 2
container_start_page 119
container_title Indian journal of clinical biochemistry
container_volume 15
creator Basu, P S
Majhi, R
Chatterjee, S
Batabyal, S K
description A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.
doi_str_mv 10.1007/BF02883739
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3453953</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1939464651</sourcerecordid><originalsourceid>FETCH-LOGICAL-c2799-2dcbc38dc630ae6cab5dd9711c93d3057bf5c7f3d603b6ca535ebc1bcc6fadcb3</originalsourceid><addsrcrecordid>eNpdkc1O3DAUha2qqAO0mz4AsrpCSKG2b5zEGyQYMRQJqYu2a8t_aYwycbAT0Lw9hoEpZeUrn-8enauD0FdKTikh9feLFWFNAzWID2ifiLosSMnYx-eZFFRQvkAHKd0SAiUp6Se0YEAJZ5zto_GXG1VUkw8DVoPF3rph8q0326_Q4qnbxOAtVvMUVNZ0sN4l7Ac8ZibTCT_4qdtx1qcQrYsJ6w3uNjaGsQvaG2xCP6-Hz2ivVX1yX17eQ_Rndfl7-aO4-Xl1vTy_KQyrhSiYNdpAY00FRLnKKM2tFTWlRoAFwmvdclO3YCsCOsscuNOGamOqVuVdOERnW99x1mtnTQ4aVS_H6NcqbmRQXv6vDL6Tf8O9hJKD4JANjl8MYribXZrk2ifj-l4NLsxJUspYBYySKqPf3qG3YY5DPk-y-smuEU2GTraQiSGl6NpdFkrkU4_yX48ZPnqbfoe-FgePbs-cVg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>274539898</pqid></control><display><type>article</type><title>Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column</title><source>Open Access: PubMed Central</source><source>Springer Nature</source><creator>Basu, P S ; Majhi, R ; Chatterjee, S ; Batabyal, S K</creator><creatorcontrib>Basu, P S ; Majhi, R ; Chatterjee, S ; Batabyal, S K</creatorcontrib><description>A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.</description><identifier>ISSN: 0970-1915</identifier><identifier>EISSN: 0974-0422</identifier><identifier>DOI: 10.1007/BF02883739</identifier><identifier>PMID: 23105252</identifier><language>eng</language><publisher>India: Springer Nature B.V</publisher><subject>Methods ; Thyroid diseases ; Thyroid gland</subject><ispartof>Indian journal of clinical biochemistry, 2000-07, Vol.15 (2), p.119-123</ispartof><rights>Association of Clinical Biochemists of India 2000</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c2799-2dcbc38dc630ae6cab5dd9711c93d3057bf5c7f3d603b6ca535ebc1bcc6fadcb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3453953/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3453953/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27923,27924,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23105252$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Basu, P S</creatorcontrib><creatorcontrib>Majhi, R</creatorcontrib><creatorcontrib>Chatterjee, S</creatorcontrib><creatorcontrib>Batabyal, S K</creatorcontrib><title>Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column</title><title>Indian journal of clinical biochemistry</title><addtitle>Indian J Clin Biochem</addtitle><description>A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.</description><subject>Methods</subject><subject>Thyroid diseases</subject><subject>Thyroid gland</subject><issn>0970-1915</issn><issn>0974-0422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNpdkc1O3DAUha2qqAO0mz4AsrpCSKG2b5zEGyQYMRQJqYu2a8t_aYwycbAT0Lw9hoEpZeUrn-8enauD0FdKTikh9feLFWFNAzWID2ifiLosSMnYx-eZFFRQvkAHKd0SAiUp6Se0YEAJZ5zto_GXG1VUkw8DVoPF3rph8q0326_Q4qnbxOAtVvMUVNZ0sN4l7Ac8ZibTCT_4qdtx1qcQrYsJ6w3uNjaGsQvaG2xCP6-Hz2ivVX1yX17eQ_Rndfl7-aO4-Xl1vTy_KQyrhSiYNdpAY00FRLnKKM2tFTWlRoAFwmvdclO3YCsCOsscuNOGamOqVuVdOERnW99x1mtnTQ4aVS_H6NcqbmRQXv6vDL6Tf8O9hJKD4JANjl8MYribXZrk2ifj-l4NLsxJUspYBYySKqPf3qG3YY5DPk-y-smuEU2GTraQiSGl6NpdFkrkU4_yX48ZPnqbfoe-FgePbs-cVg</recordid><startdate>20000701</startdate><enddate>20000701</enddate><creator>Basu, P S</creator><creator>Majhi, R</creator><creator>Chatterjee, S</creator><creator>Batabyal, S K</creator><general>Springer Nature B.V</general><general>Springer India</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>04Q</scope><scope>04W</scope><scope>3V.</scope><scope>7XB</scope><scope>88A</scope><scope>88I</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20000701</creationdate><title>Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column</title><author>Basu, P S ; Majhi, R ; Chatterjee, S ; Batabyal, S K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2799-2dcbc38dc630ae6cab5dd9711c93d3057bf5c7f3d603b6ca535ebc1bcc6fadcb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Methods</topic><topic>Thyroid diseases</topic><topic>Thyroid gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Basu, P S</creatorcontrib><creatorcontrib>Majhi, R</creatorcontrib><creatorcontrib>Chatterjee, S</creatorcontrib><creatorcontrib>Batabyal, S K</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>India Database</collection><collection>India Database: Science &amp; Technology</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Indian journal of clinical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Basu, P S</au><au>Majhi, R</au><au>Chatterjee, S</au><au>Batabyal, S K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column</atitle><jtitle>Indian journal of clinical biochemistry</jtitle><addtitle>Indian J Clin Biochem</addtitle><date>2000-07-01</date><risdate>2000</risdate><volume>15</volume><issue>2</issue><spage>119</spage><epage>123</epage><pages>119-123</pages><issn>0970-1915</issn><eissn>0974-0422</eissn><abstract>A method has been developed to separate and identify thyroglobulin autoantibody (TgAb) and thyroid peroxidase autoantibody (TPOAb) in serum obtained from normal and autoimmune thyroid diseases using phenyl Sepharose CL-4B hydrophobic column. The protein peaks obtained from hydrophobic column were identified as TgAb and TPOAb by comparing the elution profile of commercially purified standard thyroid autoantibodies. The similarity of the inhibitory effects of eluted proteins and of standard thyroid autoantibodies on lectin concanavalin A-RBC interaction confirmed the separation of TPO-Ab and TgAb by the hydrophobic, column. The eluted fractions from the hydrophobic column were estimated by the radio immunoassay (RIA) to confirm the presence of both auto-antibodies. This hydrophobic column method offers an advantage of visual inspection of this autoantibodies by graphic representation of peak height along with their estimation in autoimmune thyroid disorders.</abstract><cop>India</cop><pub>Springer Nature B.V</pub><pmid>23105252</pmid><doi>10.1007/BF02883739</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0970-1915
ispartof Indian journal of clinical biochemistry, 2000-07, Vol.15 (2), p.119-123
issn 0970-1915
0974-0422
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3453953
source Open Access: PubMed Central; Springer Nature
subjects Methods
Thyroid diseases
Thyroid gland
title Separation and identification of thyroid autoantibodies in patients with thyroid disorders by hydrophobic column
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T10%3A42%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20and%20identification%20of%20thyroid%20autoantibodies%20in%20patients%20with%20thyroid%20disorders%20by%20hydrophobic%20column&rft.jtitle=Indian%20journal%20of%20clinical%20biochemistry&rft.au=Basu,%20P%20S&rft.date=2000-07-01&rft.volume=15&rft.issue=2&rft.spage=119&rft.epage=123&rft.pages=119-123&rft.issn=0970-1915&rft.eissn=0974-0422&rft_id=info:doi/10.1007/BF02883739&rft_dat=%3Cproquest_pubme%3E1939464651%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c2799-2dcbc38dc630ae6cab5dd9711c93d3057bf5c7f3d603b6ca535ebc1bcc6fadcb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=274539898&rft_id=info:pmid/23105252&rfr_iscdi=true