Coilin levels modulate cell cycle progression and γH2AX levels in etoposide treated U2OS cells

• Published in: FEBS letters 2012-09, Vol.586 (19), p.3404-3409
• Main Authors: Velma, Venkatramreddy, Carrero, Zunamys I., Allen, Carmen B., Hebert, Michael D.
• Format: Article
• Language: English
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Cell cycle; Cell Cycle Checkpoints - drug effects; Cell Cycle Checkpoints - physiology; Cell Line, Tumor; Cell Nucleolus - drug effects; Cell Nucleolus - metabolism; Cell Proliferation - drug effects; Coiled Bodies - drug effects; Coiled Bodies - metabolism; Coilin; DNA Damage; Etoposide - pharmacology; Gene Knockdown Techniques; Histones - metabolism; Humans; Nuclear Proteins - antagonists & inhibitors; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; RNA, Small Interfering - genetics; Solubility; γH2AX
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2012.07.054

► Investigated the role of coilin in the DNA damage response. ► Depletion of coilin increased levels of γH2AX in etoposide treated cells. ► Proliferation rate of etoposide treated U2OS cells was influenced by coilin. ► Coilin overexpression in treated cells increases percent of cells in S and G2/M. ► Transfected coilin inhibits the localization of endogenous coilin in treated cells. Coilin is considered the Cajal body (CB) marker protein. In this report, we investigated the role of coilin in the DNA damage response and found that coilin reduction correlated with significantly increased levels of soluble γH2AX in etoposide treated U2OS cells. Additionally, coilin levels influenced the proliferation rate and cell cycle distribution of cells exposed to etoposide. Moreover, coilin overexpression inhibited nucleolar localization of endogenous coilin in etoposide treated U2OS cells. Collectively, these data provide additional evidence for coilin and CBs in the DNA damage response.