Chromomycin A3 staining as a useful tool for evaluation of male fertility

Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate. Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2000, Vol.17 (1), p.60-66
Main Authors: FARHAD GOLSHAN IRANPOUR, MOHAMMAD HOSEIN NASR-ESFAHANI, MOJTABA REZAZADEH VALOJERDI, TAKI MOHAMMAD TAKI AL-TARAIHI
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell physiology
Chromatin - ultrastructure
Chromomycin A3
Female
Fertilization in Vitro
Fundamental and applied biological sciences. Psychology
Humans
Infertility, Male - diagnosis
Infertility, Male - pathology
Male
Molecular and cellular biology
Motility and taxis
Sperm Count
Sperm Motility
Spermatozoa - physiology
Spermatozoa - ultrastructure
Staining and Labeling - methods
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Summary:Our purpose was to investigate the association between percentage chromomycin A3 (CMA3) positivity of spermatozoa with some sperm parameters and in vitro fertilization rate. Spermatozoa were collected from 139 men, washed in PBS, fixed in methanol/glacial acetic acid (3:1), and then spread on slides. CMA3 positivity is expressed as the percentage in 200 spermatozoa. Percentage of CMA3 positivity showed not only a negative correlation with fertilization rate but also a significant difference between fertilizing and nonfertilizing patients. Moreover, percentage of CMA3-positive spermatozoa showed a negative correlation with count and percentage motility and a positive correlation with percentage of abnormal morphology. Percentage of CMA3 positivity also had a positive correlation with some abnormalities of head such as amorphous and macrocephaly. Ultrastructural study showed chromatin unpackaging in high CMA3-positive semen samples in comparison with low CMA3-positive semen samples. There is a close relationship among fertilization rate, sperm parameters, and CMA3 positivity and CMA3 could be considered as a useful tool for evaluation of male fertility prior to infertility treatment.
ISSN:1058-0468
1573-7330
DOI:10.1023/A:1009406231811