The efficacy of test tube warming devices used during oocyte retrieval for IVF

To investigate whether commonly used test tube warming devices maintain a constant temperature in follicular fluid aspirates. By using a digital thermocouple, temperature was measured and comparisons were made between an analog dry block heater, a digital dry block heater, and a thermostatic test tu...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2004-10, Vol.21 (10), p.355-360
Main Authors: Yeung, Queenie Sum Yee, Briton-Jones, Christine May, Tjer, Grace Ching Ching, Chiu, Tony Tak Yu, Haines, Christopher
Format: Article
Language:English
Subjects:
Adult
Embryos
Equipment Design
Female
Fertilization in Vitro - methods
Heaters
Humans
In vitro fertilization
Laboratories
Oocyte Donation - methods
Pregnancy
Reproductive technologies
Specimen Handling
Temperature
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Summary:To investigate whether commonly used test tube warming devices maintain a constant temperature in follicular fluid aspirates. By using a digital thermocouple, temperature was measured and comparisons were made between an analog dry block heater, a digital dry block heater, and a thermostatic test tube heater. For small fluid volumes, temperature in the block heaters increased above 37 degrees C after being in the block for over 2 min. The thermostatic heater maintained a constant temperature, but this was below the factory setting of 36.9 degrees C. Temperature maintenance was influenced by fluid volume in each tube. One of the key factors in the handling of gametes and embryos is the maintenance of constant temperature. Test tube warming devices require verification of their ability to maintain fluid at the desired temperature. Temperature may vary with fluid volume and the type of test tube warming device used.
ISSN:1058-0468
1573-7330
DOI:10.1023/B:JARG.0000046203.44045.0e