Twin pregnancy obtained with frozen-thawed embryos after in vitro maturation in a patient with polycystic ovarian syndrome

• Published in: Journal of assisted reproduction and genetics 2003-08, Vol.20 (8), p.347-350
• Main Authors: GODIN, P.-A, GASPARD, O, THONON, F, JOUAN, C, WIJZEN, F, DUBOIS, M, FOIDART, J.-M
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Cryopreservation; embryo cryopreservation; Embryo Transfer; Embryo, Mammalian; Female; Fundamental and applied biological sciences. Psychology; Genetics & genetic processes; Génétique & processus génétiques; Human health sciences; Humans; in vitro maturation; Infertility, Female - therapy; Life sciences; Mother. Fetoplacental unit. Mammary gland. Milk; Médecine de la reproduction (Gynécologie, andrologie, obstétrique); polycystic ovarian syndrome; Polycystic Ovary Syndrome; Pregnancy; Pregnancy. Parturition. Lactation; Reproductive medicine (gynecology, andrology, obstetrics); Sciences de la santé humaine; Sciences du vivant; Sperm Injections, Intracytoplasmic; Twins; Vertebrates: reproduction
• ISSN: 1058-0468; 1573-7330; 1573-7330
• DOI: 10.1023/A:1024869826622

A twin pregnancy was obtained in a patient with polycystic ovary syndrome after the transfer of three in vitro maturation-derived day 3 embryos that has been frozen and thawed. The patient had received mild hMG stimulation followed by hCG injection. After culture for 24-48 h, mature oocytes were fertilized by ICSI. Embryos were cultured until day 3; supernumerary embryos were cryopreserved using a slow protocol. Among 15 nonatretic oocytes, 9 matured, 8 were fertilized. Four embryos were transferred but they did not implant. The subsequent transfer of three frozen-thawed embryos resulted in the delivery of two healthy girls. These results indicate that a pregnancy could be obtained with in vitro maturation-derived day-3 frozen-thawed embryos.