Pregnancy and delivery after in vitro maturation of naked ICSI-GV oocytes with GH and transfer of a frozen thawed blastocyst: case report

To determine if GV oocytes, collected at the time of ICSI, can be matured in vitro and rescued for therapeutic treatment. A patient for whom all the collected oocytes at the GV stage after a classical COH protocol were matured in vitro with GH. All the naked oocytes were matured in a culture medium...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2006-01, Vol.23 (1), p.47-49
Main Authors: Menezo, Yves J R, Nicollet, Bernard, Rollet, Jacques, Hazout, André
Format: Article
Language:English
Subjects:
Adult
Blastocyst
Case Report
Cryopreservation
Delivery, Obstetric
Embryo Transfer
Female
Growth Hormone - pharmacology
Humans
Live Birth
Oocytes - drug effects
Oocytes - transplantation
Pregnancy
Sperm Injections, Intracytoplasmic - methods
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Summary:To determine if GV oocytes, collected at the time of ICSI, can be matured in vitro and rescued for therapeutic treatment. A patient for whom all the collected oocytes at the GV stage after a classical COH protocol were matured in vitro with GH. All the naked oocytes were matured in a culture medium (ISM2) containing 15% patient serum +1.6 units of GH (Saizen) per millilitre. Oocytes were incubated overnight at 37 degrees C. The MII oocytes obtained were micro-injected. A fresh transfer was performed and a supernumerary blastocyst was frozen. The patient was pregnant and delivered a healthy girl after transfer of the frozen/thawed blastocyst. The baby girl is now 2 years old. In vitro maturation with GH allows rescuing naked GV oocytes collected at the time of ICSI. GH action does not pass through the cumulus cells. According to the possible lack of synchrony between the embryo and the uterus, we recommend to freeze the embryos obtained and to replace them in a controlled cycle.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-005-9014-0