Developing techniques for determining sperm morphology in today's andrology laboratory

To investigate three areas: the staining of spermatozoa; the Computer Assisted Semen Analysis (CASA); and the variability of technicians. Staining experiment: sperm from 15 beef bulls were randomized to one of three staining protocols. CASA experiment: slides were evaluated using the Integrated Visu...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2005-05, Vol.22 (5), p.219-225
Main Authors: Graves, Jennifer E, Higdon, 3rd, H Lee, Boone, William R, Blackhurst, Dawn W
Format: Article
Language:English
Subjects:
Adult
Andrology
Animals
Cattle
Digitization
Experiments
Hospital systems
Humans
Image Processing, Computer-Assisted
Infertility
Infertility, Male - diagnosis
Laboratories
Male
Middle Aged
Morphology
Observer Variation
Quality control
Quality standards
Random Allocation
Sperm
Spermatozoa - cytology
Spermatozoa - physiology
Staining and Labeling - methods
Stains & staining
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Summary:To investigate three areas: the staining of spermatozoa; the Computer Assisted Semen Analysis (CASA); and the variability of technicians. Staining experiment: sperm from 15 beef bulls were randomized to one of three staining protocols. CASA experiment: slides were evaluated using the Integrated Visual Optical System and compared to technician results. Variability of technicians: five laboratorians analyzed the same set of 20 slides six different times. Staining experiment: the size of the sperm increased in proportion to increased time and heat associated with each successive protocol. CASA experiment: coefficient of variation ranged from 18.3 to 101.7% (12 slides). Variability of technicians: the mean sperm morphology results ranged from 7.3 to 15% normal forms. Until laboratories adhere to the universal standard set by the World Health Organization to evaluate sperm morphology, a laboratory must rely on its own quality control to insure repeatable results.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-005-4925-3