The effect of preincubation period of oocytes on nuclear maturity, fertilization rate, embryo quality, and pregnancy outcome in IVF and ICSI

To clarify the effect of preincubation of oocytes on the results of IVF and ICSI. A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2003-09, Vol.20 (9), p.358-364
Main Authors: HO, Jason Yen-Ping, CHEN, Ming-Jer, YI, Yu-Chiao, GUU, Hwa-Fen, HO, Esther Shih-Chu
Format: Article
Language:English
Subjects:
Biological and medical sciences
Embryo, Mammalian - physiology
Female
Fertilization - physiology
Fertilization in Vitro - methods
Fundamental and applied biological sciences. Psychology
Hormone metabolism and regulation
Humans
Male
Oocytes - physiology
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Pregnancy. Parturition. Lactation
Retrospective Studies
Sperm Injections, Intracytoplasmic
Time Factors
Vertebrates: reproduction
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Summary:To clarify the effect of preincubation of oocytes on the results of IVF and ICSI. A total of 176 IVF and 64 ICSI cycles received long protocol ovarian stimulation. The oocytes were incubated for 1-8 h before insemination or sperm injection. Metaphase II (MII) percentage was evaluated in the ICSI arm; fertilization rates, embryo quality, and pregnancy outcomes were analyzed in both IVF and ICSI arms according to the preincubation period duration of oocytes. The MII percentage of the ICSI arm was significantly lower (P < 0.05) in the group with preincubation period of < 2.5 h. The fertilization rates in groups with preincubation for 2.5-5.5 h were significantly higher (P < 0.001) for IVF. Embryo quality and pregnancy outcomes were not significantly different between the IVF or ICSI arm. The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.
ISSN:1058-0468
1573-7330
DOI:10.1023/A:1025476910771