Dielectric properties of yeast cells expressed with the motor protein prestin

We report on the linear and nonlinear dielectric properties of budding yeast (S. cerevisiae) cells, one strain of which has been genetically modified to express prestin. This motor protein plays a crucial role in the large electromotility exhibited by the outer hair cells of mammalian inner ears. Li...

Full description

Saved in:
Bibliographic Details
Published in:Journal of biological physics 2005-12, Vol.31 (3-4), p.465-475
Main Authors: Miller, Jr, John H, Nawarathna, Dharmakeerthi, Warmflash, David, Pereira, Fred A, Brownell, William E
Format: Article
Language:English
Subjects:
Bacteria
Cell suspensions
Dielectric properties
Inner ear
Lipids
Membrane potential
Microbiology
outer hair cells
Probes
Saccharomyces cerevisiae
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report on the linear and nonlinear dielectric properties of budding yeast (S. cerevisiae) cells, one strain of which has been genetically modified to express prestin. This motor protein plays a crucial role in the large electromotility exhibited by the outer hair cells of mammalian inner ears. Live cell suspensions exhibit enormous dielectric responses, which can be used to probe metabolic activity, membrane potential, and other properties. The aims of this study are: (1) to compare the dielectric responses of organisms expressing prestin from those of control specimens, and (2) ultimately to further develop dielectric response as a tool to study live cells, proteins, and lipids.
ISSN:0092-0606
1573-0689
DOI:10.1007/s10867-005-6064-6