RNA intermediates in potato spindle tuber viroid replication

Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA· RNA hybridization techniques. These replicative intermediates contain monomeric circular or l...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982, Vol.79 (1), p.113-117
Main Authors: Owens, Robert A., Diener, T. O.
Format: Article
Language:English
Subjects:
Biological Sciences: Cell Biology
Circles
DNA probes
Electrophoresis
Gels
Molecules
Phenols
potato spindle tuber viroid
RNA
Tubers
Viroids
Virology
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Summary:Two double-stranded RNA intermediates of viroid replication have been isolated from potato spindle tuber viroid (PSTV)-infected tomato tissue and characterized by polyacrylamide gel electrophoresis and DNA· RNA hybridization techniques. These replicative intermediates contain monomeric circular or linear PSTV strands complexed with a multimeric complementary RNA strand. Synchronous synthesis of single-stranded PSTV is accompanied by a simultaneous marked increase in double-stranded PSTV RNA; thus, in vivo precursors of the characterized double-stranded PSTV RNAs seem to be involved in PSTV replication. A ``rolling circle'' model for viroid replication on a circular PSTV template can accommodate the double-stranded PSTV RNA species characterized.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.1.113