Video-Rate Molecular Imaging in Vivo with Stimulated Raman Scattering

Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy witho...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2010-12, Vol.330 (6009), p.1368-1370
Main Authors: Saar, Brian G, Freudiger, Christian W, Reichman, Jay, Stanley, C. Michael, Holtom, Gary R, Xie, X. Sunney
Format: Article
Language:English
Subjects:
Administration, Cutaneous
Animals
Backscattering
Biocompatibility
Biological and medical sciences
Biomedical materials
Capillaries
Dimethyl Sulfoxide - administration & dosage
Dimethyl Sulfoxide - pharmacokinetics
Epidermis
Epidermis - chemistry
Epidermis - metabolism
Erythrocytes - physiology
Hair
Human
Humans
Imaging
Imaging, Three-Dimensional
In vivo testing
In vivo tests
Investigative techniques, diagnostic techniques (general aspects)
Laser beams
Light
Lipids
Magnetic resonance imaging
Male
Medical sciences
Mice
Mice, Nude
Microscopy
Miscellaneous. Technology
Molecular Imaging - methods
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pumps
Raman scattering
Skin
Skin - blood supply
Skin - chemistry
Skin - metabolism
Spectrum Analysis, Raman - methods
Surgical implants
Time Factors
Vitamin A - administration & dosage
Vitamin A - pharmacokinetics
Water
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Summary:Optical imaging in vivo with molecular specificity is important in biomedicine because of its high spatial resolution and sensitivity compared with magnetic resonance imaging. Stimulated Raman scattering (SRS) microscopy allows highly sensitive optical imaging based on vibrational spectroscopy without adding toxic or perturbative labels. However, SRS imaging in living animals and humans has not been feasible because light cannot be collected through thick tissues, and motion-blur arises from slow imaging based on backscattered light. In this work, we enable in vivo SRS imaging by substantially enhancing the collection of the backscattered signal and increasing the imaging speed by three orders of magnitude to video rate. This approach allows label-free in vivo imaging of water, lipid, and protein in skin and mapping of penetration pathways of topically applied drugs in mice and humans.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1197236