Identification of Cytokeratin Antigens in Novikoff Ascites Hepatoma

Further characterization of three polypeptide antigens present in Novikoff ascites hepatoma (molecular weights 39,000, 49,000, and 56,000--p39, p49, and p56, respectively), has established the identity of these proteins as cytokeratins. Two-dimensional gel electrophoresis revealed that each of the t...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1982-05, Vol.79 (10), p.3138-3142
Main Authors: Schmidt, Warren N., Pardue, Robert L., Tutt, Michele C., Briggs, Robert C., Brinkley, B. R., Hnilica, L. S.
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - analysis
Animals
Antibodies
Antigens
Antigens, Neoplasm - analysis
B lymphocytes
cell lines
Cellulose nitrate
Cytoskeleton - immunology
Epithelial cells
Fluorescent Antibody Technique
Gels
Hepatocellular carcinoma
hepatoma
keratin
Keratins
Keratins - immunology
Liver
Liver Neoplasms, Experimental - immunology
Male
Molecular Weight
purification
Rats
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Summary:Further characterization of three polypeptide antigens present in Novikoff ascites hepatoma (molecular weights 39,000, 49,000, and 56,000--p39, p49, and p56, respectively), has established the identity of these proteins as cytokeratins. Two-dimensional gel electrophoresis revealed that each of the three proteins exists in several isoelectric forms, all characteristic of the keratins. The antigens p39 and p56 had amino acid compositions compatible with this family of proteins, and immunofluorescence microscopy localized the antigens on elaborate filament arrays indicative of the cytokeratins. Antibodies monospecific for the p39 antigen, purified by using affinity chromatography, were used to demonstrate that protein p39 was specific for Novikoff hepatoma cells. These results suggest that the cytokeratins contain cell-specific species that can be useful as markers for differentiation and neoplasia.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.79.10.3138