Effect of Galla chinensis on the remineralization of two bovine root lesions morphous in vitro

• Published in: International journal of oral science 2012-09, Vol.4 (3), p.152-156
• Main Authors: Guo, Bin, Que, Ke-Hua, Yang, Jing, Wang, Bo, Liang, Qian-Qian, Xie, Hong-Hui
• Format: Article
• Language: English
• Subjects: Animals; Cariostatic Agents - therapeutic use; Cattle; Dentin - pathology; Dentistry; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - therapeutic use; Gallic Acid - therapeutic use; Hydrogen-Ion Concentration; Hydrolyzable Tannins - therapeutic use; Medicine; Microscopy, Confocal; Microscopy, Polarization; Oral and Maxillofacial Surgery; Original; original-article; Orthopedics; Polyphenols - therapeutic use; Random Allocation; Root Caries - drug therapy; Surgical Orthopedics; Tooth Remineralization; 五倍子; 体外; 形态; 水溶液处理; 激光扫描共聚焦显微镜; 牛; 病变; 矿化作用
• ISSN: 1674-2818; 2049-3169
• DOI: 10.1038/ijos.2012.45

The present study aims to evaluate the effect of Galla chinensis compounds on the remineralization of two artificial root lesions morphous in vitro. Sixty bovine dentine blocks were divided into two groups and individually treated with two levels of demineralization solutions to form erosive and subsurface artificial carious lesions in vitro. Each group was then divided into three subgroups, each of which were treated with a remineralization solution （positive control）, deionized water （negative control）, or 4 000 mg-L-1 aqueous solutions of Galla chinensis extract. The dentine blocks were then subjected to a pH-cycling regime for 7 days. During the first 4 days, the daily cycle included 21-h deal and 3-h demineralization applications. The dentine blocks were dealt with the entire day during the remaining 3 days. Two specimens from each of the treatment groups were selected and observed under a polarized light microscope. Data collected using a laser scanning confocal microscope were computerized and analyzed. Galla chinensis extract clearly enhanced the remineralization of both erosive lesion and subsurface lesion patterns in the specimens （P〈0.05）. The level of remineralization of the erosive lesion by Galla chinensis extract was lower than that of the subsurface lesion （P〈0.05）. In addition, the remineralization of the subsurface lesion by Galla chinensis extract was higher than that of the remineralization solution （P〈0.05）. No significant difference between the remineralization of erosive lesions by Galla chinensis extract and the remineralization solution was observed （P〉0.05）. So Galla chinensis extract has the potential to improve the remineralization of artificial root lesions under dynamic pH-cyclic conditions, indicating its potential use as a natural remineralization medicine.