In vivo gene transfer of leukemia inhibitory factor (LIF) into mouse endometrium

Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium. Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Gro...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2002-02, Vol.19 (2), p.79-83
Main Authors: HSIEH, Yao-Yuan, LIN, Chich-Sheng, SUN, Yu-Ling, CHANG, Chi-Chen, TSAI, Horng-Der, WU, Jackson Chieh-Hsi
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Cloning
Cytokines
Cytomegalovirus
Dehydrogenases
DNA, Complementary
Endometrium
Endometrium - physiology
Female
Females
Fundamental and applied biological sciences. Psychology
Gene expression
Gene Transfer Techniques
Genetic engineering
Genetic technics
Genetic Vectors - genetics
Glyceraldehyde-3-Phosphate Dehydrogenases - genetics
Growth Inhibitors - genetics
Interleukin-6
Laparotomy
Leukemia
Leukemia Inhibitory Factor
Liposomes
Luciferases - genetics
Lymphokines - genetics
Methods. Procedures. Technologies
Mice
Mice, Inbred ICR
Plasmids
Uterus
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:Leukemia inhibitory factor (LIF) is important for embryogenesis and implantation. We aimed to transfect LIF gene into the mouse endometrium. Expression plasmids carried LIF and luciferase genes for transfer. After superovulation, 100 ICR mice were mated with vasectomized mice. Then LIF-liposome (Group 1) and luciferase-liposome complexes (Group 2) were injected into their uterine lumen (Day 0). Endometrial LIF and luciferase expressions were detected by reverse transcription-polymerase chain reaction on Days 0-4 post gene transfer. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as an internal control to normalize the gene transfection. LIF mRNA and luciferase activities reached the peak expression on Day 3. In Group 1, the ratios of LIF/GADPH on Days 1-4 were 0.414, 1.096, 1.162, and 0.782. In Group 2. LIF/GADPH on Days 1-4 were 0.24, 0.22, 0.35, and 0.32. Mouse endometrium could be effectively transfected with liposome-DNA mixtures. Endometrial LIF transfer via liposome may be effective in human.
ISSN:1058-0468
1573-7330
DOI:10.1023/A:1014495614792