Prognostic implication of apoptosis in human luteinized granulosa cells during IVF-ET

To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET. Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibod...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2002-05, Vol.19 (5), p.209-214
Main Authors: Suh, Chang Suk, Jee, Byung Chul, Choi, Young Min, Kim, Jung Gu, Lee, Jin Yong, Moon, Shin Yong, Kim, Seok Hyun
Format: Article
Language:English
Subjects:
Adult
Antibodies, Monoclonal - pharmacology
Apoptosis
Apoptosis - physiology
Biological and medical sciences
Cell cycle
Embryo Transfer
Embryos
Estradiol - blood
Female
Fertilization in Vitro
Flow Cytometry
Follicle Stimulating Hormone - blood
Follicles
Fundamental and applied biological sciences. Psychology
Granulosa Cells - cytology
Granulosa Cells - physiology
Humans
Linear Models
Male
Monoclonal antibodies
Mother. Fetoplacental unit. Mammary gland. Milk
Ovaries
Patients
Predictive Value of Tests
Pregnancy
Pregnancy. Parturition. Lactation
Prognosis
Propidium - pharmacology
ROC Curve
Vertebrates: reproduction
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Summary:To investigate the prognostic significance of apoptosis in granulosa cells recovered from patients participating in IVF-ET. Seventy-three women underwent ovarian hyperstimulation for IVF and embryo transfer. After follicle aspiration, recovered granulosa cells were stained by BCL2 monoclonal antibody with FITC and propidium iodide (PI). Fluorescence was detected by flowcytometry, then the apoptotic index (Al) and BCL2 positivity were assessed. The pregnant group showed a significantly lower apoptotic index in granulosa cells compared with the nonpregnant group (p < 0.0001). Patient's age, basal serum FSH concentration, serum E2 concentrations at hCG day, number of retrieved oocytes, fertilization rates. number of embryos transferred, and BCL2 positivity were not different between the two groups. Linear regression analysis of AI to serum basal FSH showed a positive correlation. Al in granulosa cells of 6.14% or below could predict a successful pregnancy with a sensitivity of 87.5% and a specificity of 73.7%. Our results indicate that apoptotic analysis within granulosa cells can be used as a prognostic indicator for IVF success.
ISSN:1058-0468
1573-7330
DOI:10.1023/A:1015319617598