Synthetic polymer coatings for long-term growth of human embryonic stem cells

We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The developmen...

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Bibliographic Details
Published in:Nature biotechnology 2010-06, Vol.28 (6), p.581-583
Main Authors: Lahann, Joerg, Smith, Gary D, Villa-Diaz, Luis G, Nandivada, Himabindu, Ding, Jun, Nogueira-de-Souza, Naiara C, Krebsbach, Paul H, O'Shea, K Sue
Format: Article
Language:English
Subjects:
631/532/2117
631/61/2320
631/61/338/552
631/80/641/83
Agriculture
Ammonium
Animals
Bioinformatics
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
brief-communication
Cell Culture Techniques - methods
Cell Proliferation - drug effects
Cells, Cultured
Coated Materials, Biocompatible - pharmacology
Culture media
Culture Media - pharmacology
Embryonic stem cells
Embryonic Stem Cells - cytology
Embryonic Stem Cells - drug effects
Embryos
Fibroblasts - cytology
Fibroblasts - drug effects
Fundamental and applied biological sciences. Psychology
Health. Pharmaceutical industry
Humans
Industrial applications and implications. Economical aspects
Life Sciences
Mice
Miscellaneous
Physiological aspects
Polymers
Polymers - pharmacology
Stem cells
Time Factors
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Description
Summary:We report a fully defined synthetic polymer coating, poly[2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl)ammonium hydroxide] (PMEDSAH), which sustains long-term human embryonic stem (hES) cell growth in several different culture media, including commercially available defined media. The development of a standardized, controllable and sustainable culture matrix for hES cells is an essential step in elucidating mechanisms that control hES cell behavior and in optimizing conditions for biomedical applications of hES cells.
ISSN:1087-0156
1546-1696
DOI:10.1038/nbt.1631