Oleic Acid Attenuates trans-10,cis-12 Conjugated Linoleic Acid-Mediated Inflammatory Gene Expression in Human Adipocytes

• Published in: Lipids 2012-11, Vol.47 (11), p.1043-1051
• Main Authors: Reardon, Meaghan, Gobern, Semone, Martinez, Kristina, Shen, Wan, Reid, Tanya, McIntosh, Michael
• Format: Article
• Language: English
• Subjects: Adipocytes; Adipocytes - drug effects; Adipocytes - metabolism; Adult; Biomedical and Life Sciences; Cells, Cultured; Conjugated linoleic acid; Dietary Supplements; dose response; Dose-Response Relationship, Drug; Fatty Acids, Monounsaturated - antagonists & inhibitors; Female; G-protein coupled receptors; gene expression; Gene Expression - drug effects; glucose; G‐protein receptors; Humans; inflammation; Inflammation - drug therapy; Inflammation - genetics; Inflammation - metabolism; Inflammatory gene expression; insulin resistance; isomers; Life Sciences; Linoleic Acids, Conjugated - administration & dosage; Linoleic Acids, Conjugated - pharmacology; lipid content; Lipidology; Medical Biochemistry; Medicinal Chemistry; Microbial Genetics and Genomics; Middle Aged; Monounsaturated fatty acids; Neurochemistry; Nutrition; Oleic acid; Oleic Acids - pharmacology; Original Article; phospholipids; stearic acid; Stearoyl-CoA Desaturase - antagonists & inhibitors; Stearoyl-CoA Desaturase - metabolism; Stearoyl‐CoA desaturase; Structure-Activity Relationship; weight loss; Young Adult
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-012-3711-0

The weight loss supplement conjugated linoleic acid (CLA) consists of an equal mixture of trans -10, cis -12 (10,12) and cis -9, trans -11 (9,11) isomers. However, high levels of mixed CLA isomers, or the 10,12 isomer, causes chronic inflammation, lipodystrophy, or insulin resistance. We previously demonstrated that 10,12 CLA decreases de novo lipid synthesis along with the abundance and activity of stearoyl-CoA desaturase (SCD)-1, a δ-9 desaturase essential for the synthesis of monounsaturated fatty acids (MUFA). Thus, we hypothesized that the 10,12 CLA-mediated decrease in SCD-1, with the subsequent decrease in MUFA, was responsible for the observed effects. To test this hypothesis, 10,12 CLA-treated human adipocytes were supplemented with oleic acid for 12 h to 7 days, and inflammatory gene expression, insulin-stimulated glucose uptake, and lipid content were measured. Oleic acid reduced inflammatory gene expression in a dose-dependent manner, and restored the lipid content of 10,12 CLA-treated adipocytes without improving insulin-stimulated glucose uptake. In contrast, supplementation with stearic acid, a substrate for SCD-1, or 9,11 CLA did not prevent inflammatory gene expression by 10,12 CLA. Notably, 10,12 CLA impacted the expression of several G-protein coupled receptors that was attenuated by oleic acid. Collectively, these data show that oleic acid attenuates 10,12 CLA-induced inflammatory gene expression and lipid content, possibly by alleviating cell stress caused by the inhibition of MUFA needed for phospholipid and neutral lipid synthesis.