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Syndecan‐2 can promote clearance of T‐cell receptor/CD3 from the cell surface
Summary T cells express the heparan sulphate proteoglycans syndecan‐2 and syndecan‐4. Syndecan‐4 plays a T‐cell inhibitory role; however, the function of syndecan‐2 is unknown. In an attempt to examine this function, syndecan‐2 was expressed constitutively in Jurkat T cells. Interestingly, the expre...
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| Published in: | Immunology 2012-11, Vol.137 (3), p.214-225 |
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| Main Authors: | , , , , |
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| Language: | English |
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| container_end_page | 225 |
| container_issue | 3 |
| container_start_page | 214 |
| container_title | Immunology |
| container_volume | 137 |
| creator | Rovira‐Clavé, Xavier Angulo‐Ibáñez, Maria Noguer, Oriol Espel, Enric Reina, Manuel |
| description | Summary
T cells express the heparan sulphate proteoglycans syndecan‐2 and syndecan‐4. Syndecan‐4 plays a T‐cell inhibitory role; however, the function of syndecan‐2 is unknown. In an attempt to examine this function, syndecan‐2 was expressed constitutively in Jurkat T cells. Interestingly, the expression of syndecan‐2 decreased the surface levels of T‐cell receptor (TCR)/CD3 complex, concomitant with intracellular retention of CD3ε and partial degradation of the TCR‐ζ chain. Immunofluorescence microscopy revealed that intracellular CD3ε co‐located with Rab‐4 endosomes. However, the intracellular pool of CD3ε did not recycle to the cell surface. The lower TCR/CD3 surface levels caused by syndecan‐2 led to reduced TCR/CD3 responsiveness. We show that the cytosolic PDZ‐binding domain of syndecan‐2 is not necessary to elicit TCR/CD3 down‐regulation. These results identify a previously unrecognized means of controlling surface TCR/CD3 expression by syndecan‐2. |
| doi_str_mv | 10.1111/j.1365-2567.2012.03626.x |
| format | article |
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T cells express the heparan sulphate proteoglycans syndecan‐2 and syndecan‐4. Syndecan‐4 plays a T‐cell inhibitory role; however, the function of syndecan‐2 is unknown. In an attempt to examine this function, syndecan‐2 was expressed constitutively in Jurkat T cells. Interestingly, the expression of syndecan‐2 decreased the surface levels of T‐cell receptor (TCR)/CD3 complex, concomitant with intracellular retention of CD3ε and partial degradation of the TCR‐ζ chain. Immunofluorescence microscopy revealed that intracellular CD3ε co‐located with Rab‐4 endosomes. However, the intracellular pool of CD3ε did not recycle to the cell surface. The lower TCR/CD3 surface levels caused by syndecan‐2 led to reduced TCR/CD3 responsiveness. We show that the cytosolic PDZ‐binding domain of syndecan‐2 is not necessary to elicit TCR/CD3 down‐regulation. These results identify a previously unrecognized means of controlling surface TCR/CD3 expression by syndecan‐2.</description><identifier>ISSN: 0019-2805</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1111/j.1365-2567.2012.03626.x</identifier><identifier>PMID: 22881146</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>CD3 Complex - immunology ; Down-Regulation ; endocytosis ; Humans ; Jurkat Cells ; Original ; Receptors, Antigen, T-Cell - immunology ; syndecan ; Syndecan-2 - immunology ; T cell receptors ; T lymphocytes ; T‐cell receptor down‐regulation ; T‐cell receptor ζ chain</subject><ispartof>Immunology, 2012-11, Vol.137 (3), p.214-225</ispartof><rights>2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd</rights><rights>2012 The Authors. Immunology © 2012 Blackwell Publishing Ltd.</rights><rights>Copyright © 2012 Blackwell Publishing Ltd</rights><rights>Copyright © 2012 Blackwell Publishing Ltd 2012</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482679/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3482679/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,724,777,781,882,27905,27906,53772,53774</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22881146$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rovira‐Clavé, Xavier</creatorcontrib><creatorcontrib>Angulo‐Ibáñez, Maria</creatorcontrib><creatorcontrib>Noguer, Oriol</creatorcontrib><creatorcontrib>Espel, Enric</creatorcontrib><creatorcontrib>Reina, Manuel</creatorcontrib><title>Syndecan‐2 can promote clearance of T‐cell receptor/CD3 from the cell surface</title><title>Immunology</title><addtitle>Immunology</addtitle><description>Summary
T cells express the heparan sulphate proteoglycans syndecan‐2 and syndecan‐4. Syndecan‐4 plays a T‐cell inhibitory role; however, the function of syndecan‐2 is unknown. In an attempt to examine this function, syndecan‐2 was expressed constitutively in Jurkat T cells. Interestingly, the expression of syndecan‐2 decreased the surface levels of T‐cell receptor (TCR)/CD3 complex, concomitant with intracellular retention of CD3ε and partial degradation of the TCR‐ζ chain. Immunofluorescence microscopy revealed that intracellular CD3ε co‐located with Rab‐4 endosomes. However, the intracellular pool of CD3ε did not recycle to the cell surface. The lower TCR/CD3 surface levels caused by syndecan‐2 led to reduced TCR/CD3 responsiveness. We show that the cytosolic PDZ‐binding domain of syndecan‐2 is not necessary to elicit TCR/CD3 down‐regulation. These results identify a previously unrecognized means of controlling surface TCR/CD3 expression by syndecan‐2.</description><subject>CD3 Complex - immunology</subject><subject>Down-Regulation</subject><subject>endocytosis</subject><subject>Humans</subject><subject>Jurkat Cells</subject><subject>Original</subject><subject>Receptors, Antigen, T-Cell - immunology</subject><subject>syndecan</subject><subject>Syndecan-2 - immunology</subject><subject>T cell receptors</subject><subject>T lymphocytes</subject><subject>T‐cell receptor down‐regulation</subject><subject>T‐cell receptor ζ chain</subject><issn>0019-2805</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqFkstO3TAQhi1UBIfLK1SW2HSTYI8Tx15QqTrQggSqKmBtGWdScpQTp04CnF0fgWfsk-BwOSrd1Jvx6P80-udCCOUs5fEdLlIuZJ5ALosUGIeUCQkyfdggs7XwgcwY4zoBxfJtstP3i5gKludbZBtAKc4zOSM_Lldtic62f34_Ao2RdsEv_YDUNWiDbR1SX9GrKDtsGhrQYTf4cDg_FrSKKB1uIztJ_Rgq63CPbFa26XH_Ne6S668nV_PT5Pz7t7P5l_OkywSXCWBWuQy05kKpopJKo2RY2kqBLdyNsCUwqBxKzrXTpS24jbLSLvZTMpBil3x-qduNN0ssHbZDsI3pQr20YWW8rc17pa1vzU9_Z0SmQBY6Fvj0WiD4XyP2g1nW_dSJbdGPvYmDFhAnVRT_R5kCEDLLJ1sH_6ALP4Y2TsLwQkolhNIqUh__Nr92_baYCBy9APd1g6u1ztlki5uFmfZspj2b6QDM8wGYB3N2cTH9xBOraqRk</recordid><startdate>201211</startdate><enddate>201211</enddate><creator>Rovira‐Clavé, Xavier</creator><creator>Angulo‐Ibáñez, Maria</creator><creator>Noguer, Oriol</creator><creator>Espel, Enric</creator><creator>Reina, Manuel</creator><general>Wiley Subscription Services, Inc</general><general>Blackwell Science Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7QR</scope><scope>7T5</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>201211</creationdate><title>Syndecan‐2 can promote clearance of T‐cell receptor/CD3 from the cell surface</title><author>Rovira‐Clavé, Xavier ; Angulo‐Ibáñez, Maria ; Noguer, Oriol ; Espel, Enric ; Reina, Manuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p4316-2e4fc429913887f689e60edaf82a7cb3ad202fce6119c9da71a0ed89c280d0263</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>CD3 Complex - immunology</topic><topic>Down-Regulation</topic><topic>endocytosis</topic><topic>Humans</topic><topic>Jurkat Cells</topic><topic>Original</topic><topic>Receptors, Antigen, T-Cell - immunology</topic><topic>syndecan</topic><topic>Syndecan-2 - immunology</topic><topic>T cell receptors</topic><topic>T lymphocytes</topic><topic>T‐cell receptor down‐regulation</topic><topic>T‐cell receptor ζ chain</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rovira‐Clavé, Xavier</creatorcontrib><creatorcontrib>Angulo‐Ibáñez, Maria</creatorcontrib><creatorcontrib>Noguer, Oriol</creatorcontrib><creatorcontrib>Espel, Enric</creatorcontrib><creatorcontrib>Reina, Manuel</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rovira‐Clavé, Xavier</au><au>Angulo‐Ibáñez, Maria</au><au>Noguer, Oriol</au><au>Espel, Enric</au><au>Reina, Manuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Syndecan‐2 can promote clearance of T‐cell receptor/CD3 from the cell surface</atitle><jtitle>Immunology</jtitle><addtitle>Immunology</addtitle><date>2012-11</date><risdate>2012</risdate><volume>137</volume><issue>3</issue><spage>214</spage><epage>225</epage><pages>214-225</pages><issn>0019-2805</issn><eissn>1365-2567</eissn><abstract>Summary
T cells express the heparan sulphate proteoglycans syndecan‐2 and syndecan‐4. Syndecan‐4 plays a T‐cell inhibitory role; however, the function of syndecan‐2 is unknown. In an attempt to examine this function, syndecan‐2 was expressed constitutively in Jurkat T cells. Interestingly, the expression of syndecan‐2 decreased the surface levels of T‐cell receptor (TCR)/CD3 complex, concomitant with intracellular retention of CD3ε and partial degradation of the TCR‐ζ chain. Immunofluorescence microscopy revealed that intracellular CD3ε co‐located with Rab‐4 endosomes. However, the intracellular pool of CD3ε did not recycle to the cell surface. The lower TCR/CD3 surface levels caused by syndecan‐2 led to reduced TCR/CD3 responsiveness. We show that the cytosolic PDZ‐binding domain of syndecan‐2 is not necessary to elicit TCR/CD3 down‐regulation. These results identify a previously unrecognized means of controlling surface TCR/CD3 expression by syndecan‐2.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>22881146</pmid><doi>10.1111/j.1365-2567.2012.03626.x</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Immunology, 2012-11, Vol.137 (3), p.214-225 |
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| language | eng |
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| source | Open Access: PubMed Central; Wiley |
| subjects | CD3 Complex - immunology Down-Regulation endocytosis Humans Jurkat Cells Original Receptors, Antigen, T-Cell - immunology syndecan Syndecan-2 - immunology T cell receptors T lymphocytes T‐cell receptor down‐regulation T‐cell receptor ζ chain |
| title | Syndecan‐2 can promote clearance of T‐cell receptor/CD3 from the cell surface |
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