Differential expression of alternative oxidase genes in soybean cotyledons during postgerminative development

The expression of the alternative oxidase (AOX) was investigated during cotyledon development in soybean (Glycine max [L.] Merr.) seedlings. The total amount ofAOX protein increased throughout development, not just in earlier stages as previously thought, and was correlated with the increase in capa...

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Bibliographic Details
Published in:Plant physiology (Bethesda) 1998-10, Vol.118 (2), p.675-682
Main Authors: McCabe, T.C, Finnegan, P.M, Millar, A.H, Day, D.A, Whelan, J
Format: Article
Language:English
Subjects:
age
Agronomy. Soil science and plant productions
biochemical pathways
Biochemistry and Macromolecular Structure
Biological and medical sciences
Classical and quantitative genetics. Population genetics. Molecular genetics
Cotyledons
Developmental biology
developmental stages
enzyme activity
Fundamental and applied biological sciences. Psychology
Gene expression
Gene expression regulation
Generalities. Genetics. Plant material
Genes
genetic regulation
Genetics and breeding of economic plants
Glycine max
isozymes
Mitochondria
Molecular and cellular biology
Molecular genetics
Oxidases
oxidoreductases
Plants
protein composition
protein content
protein synthesis
RNA
seed development
seed germination
Seedlings
Soybeans
ubiquinones
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Summary:The expression of the alternative oxidase (AOX) was investigated during cotyledon development in soybean (Glycine max [L.] Merr.) seedlings. The total amount ofAOX protein increased throughout development, not just in earlier stages as previously thought, and was correlated with the increase in capacity of the alternative pathway. Each AOX isoform (AOX1, AOX2, and AOX3) showed a different developmental trend in mRNA abundance, such that the increase in AOX protein and capacity appears to involve a shift in gene expression from AOX2 to AOX3. As the cotyledons aged, the size of the mitochondrial ubiquinone pool decreased. We discuss how this and other factors may affect the alternative pathway activity that results from the developmental regulation of AOX expression.
ISSN:0032-0889
1532-2548
DOI:10.1104/pp.118.2.675