Deoxyribopyrimidine Triphosphatase Activity Specific for Cells Infected with Herpes Simplex Virus Type 1

Nuclei from baby hamster kidney cells infected with herpes simplex virus type 1 contain a virus-specific deoxyribonucleoside triphosphate degrading activity. The reaction proceeds at 4 degrees C and can thus be distinguished from host enzymes. Under these conditions the enzyme is specific for deoxyr...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1980-04, Vol.77 (4), p.1872-1876
Main Authors: Wohlrab, Franz, Francke, Bertold
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Cell Line
Cell nucleus
Cell Nucleus - enzymology
Cricetinae
Diphosphates
Enzymes
Human herpesvirus 1
Infections
Magnesium - metabolism
Manganese - metabolism
Nucleosides
Phosphates
Pyrophosphatases - metabolism
Radioactive decay
Simplexvirus - enzymology
Substrate Specificity
Viral DNA
Viruses
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Summary:Nuclei from baby hamster kidney cells infected with herpes simplex virus type 1 contain a virus-specific deoxyribonucleoside triphosphate degrading activity. The reaction proceeds at 4 degrees C and can thus be distinguished from host enzymes. Under these conditions the enzyme is specific for deoxyribopyrimidine triphosphates and catalyzes pyrophosphate cleavage to produce the monophosphates, dUTP being the best substrate followed by dCTP and dTTP. The appearance of the activity after infection parallels that of viral DNA-synthesis-related functions. Of a series of eight temperature-sensitive mutants tested, two (tsD and tsK) exhibit significantly decreased triphosphatase levels after infection at nonpermissive temperature, whereas a viral deoxypyrimidine kinase-deficient mutant induced wild-type levels.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.77.4.1872