Breakthrough in chloroplast genetic engineering of agronomically important crops

Chloroplast genetic engineering offers several unique advantages, including high-level transgene expression, multi-gene engineering in a single transformation event and transgene containment by maternal inheritance, as well as a lack of gene silencing, position and pleiotropic effects and undesirabl...

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Bibliographic Details
Published in:Trends in biotechnology (Regular ed.) 2005-05, Vol.23 (5), p.238-245
Main Authors: Daniell, Henry, Kumar, Shashi, Dufourmantel, Nathalie
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Chloroplasts
Cholera
Cloning
Crops
Crops, Agricultural - genetics
Daucus carota
Deoxyribonucleic acid
DNA
Embryonic growth stage
Flowers & plants
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic engineering
Genetic Engineering - methods
Genetic Engineering - trends
Genetic technics
Genomes
Glycine max
Gossypium hirsutum
Methods. Procedures. Technologies
Nicotiana tabacum
Plants, Genetically Modified - genetics
Plastids
Plastids - genetics
Proteins
R&D
Research & development
Salinity
Salt
Soybeans
Tobacco
Transgenes
Transgenic animals and transgenic plants
Transgenic plants
Vaccines
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Summary:Chloroplast genetic engineering offers several unique advantages, including high-level transgene expression, multi-gene engineering in a single transformation event and transgene containment by maternal inheritance, as well as a lack of gene silencing, position and pleiotropic effects and undesirable foreign DNA. More than 40 transgenes have been stably integrated and expressed using the tobacco chloroplast genome to confer desired agronomic traits or express high levels of vaccine antigens and biopharmaceuticals. Despite such significant progress, this technology has not been extended to major crops. However, highly efficient soybean, carrot and cotton plastid transformation has recently been accomplished through somatic embryogenesis using species-specific chloroplast vectors. This review focuses on recent exciting developments in this field and offers directions for further research and development.
ISSN:0167-7799
1879-3096
DOI:10.1016/j.tibtech.2005.03.008