Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device

Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the...

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Bibliographic Details
Published in:Scientific reports 2012-11, Vol.2 (1), p.791, Article 791
Main Authors: Fanzio, Paola, Manneschi, Chiara, Angeli, Elena, Mussi, Valentina, Firpo, Giuseppe, Ceseracciu, Luca, Repetto, Luca, Valbusa, Ugo
Format: Article
Language:English
Subjects:
631/57
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639/925/350
639/925/927/351
Bacteriophage lambda - chemistry
Biosensing Techniques
Compression
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - ultrastructure
DNA sequencing
Elastomers
Humanities and Social Sciences
multidisciplinary
Nanopores - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
Polymers
Science
Sequence Analysis, DNA - methods
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Summary:Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation and is promising for further development in sequencing technology.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep00791