Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device

Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2012-11, Vol.2 (1), p.791, Article 791
Main Authors: Fanzio, Paola, Manneschi, Chiara, Angeli, Elena, Mussi, Valentina, Firpo, Giuseppe, Ceseracciu, Luca, Repetto, Luca, Valbusa, Ugo
Format: Article
Language:English
Subjects:
631/57
639/766/25
639/925/350
639/925/927/351
Bacteriophage lambda - chemistry
Biosensing Techniques
Compression
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - ultrastructure
DNA sequencing
Elastomers
Humanities and Social Sciences
multidisciplinary
Nanopores - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
Polymers
Science
Sequence Analysis, DNA - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593
cites cdi_FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593
container_end_page
container_issue 1
container_start_page 791
container_title Scientific reports
container_volume 2
creator Fanzio, Paola
Manneschi, Chiara
Angeli, Elena
Mussi, Valentina
Firpo, Giuseppe
Ceseracciu, Luca
Repetto, Luca
Valbusa, Ugo
description Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation and is promising for further development in sequencing technology.
doi_str_mv 10.1038/srep00791
format article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3494361</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1897401492</sourcerecordid><originalsourceid>FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593</originalsourceid><addsrcrecordid>eNplkUtLAzEUhYMottQu_AMy4EphNK95ZCOU1hfYKtiuQybJtFOmSU06hf57I1NLxWSRkPNx7sm9AFwieIcgye-902sIM4ZOQBdDmsSYYHx6dO-AvvdLGFaCGUXsHHQwQTQhKOmC2diqphabysyj0WQQTZ0wvrYyvFgTFbtIRENrNs7WtVbRSJfWrVrNlkH7GI0_o4kwVi6EMboOxLaS-gKclaL2ur8_e2D29DgdvsRv78-vw8FbLBNIN3EhoJJKScKUwKnEaa5KiSDOZFkwmRZYZanOoCrC78LOcp3LQmeIKpYLnTDSAw-t77opVlpJHZKKmq9dtRJux62o-F_FVAs-t1tOKKMkRcHgem_g7Fej_YYvbeNMyMxRzjIKEWU4UDctJZ31od_loQKC_GcI_DCEwF4dRzqQvy0PwG0L-CCZuXZHJf-5fQMoKZFy</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1897401492</pqid></control><display><type>article</type><title>Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device</title><source>Publicly Available Content Database</source><source>Full-Text Journals in Chemistry (Open access)</source><source>PubMed Central</source><source>Springer Nature - nature.com Journals - Fully Open Access</source><creator>Fanzio, Paola ; Manneschi, Chiara ; Angeli, Elena ; Mussi, Valentina ; Firpo, Giuseppe ; Ceseracciu, Luca ; Repetto, Luca ; Valbusa, Ugo</creator><creatorcontrib>Fanzio, Paola ; Manneschi, Chiara ; Angeli, Elena ; Mussi, Valentina ; Firpo, Giuseppe ; Ceseracciu, Luca ; Repetto, Luca ; Valbusa, Ugo</creatorcontrib><description>Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation and is promising for further development in sequencing technology.</description><identifier>ISSN: 2045-2322</identifier><identifier>EISSN: 2045-2322</identifier><identifier>DOI: 10.1038/srep00791</identifier><identifier>PMID: 23145315</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>631/57 ; 639/766/25 ; 639/925/350 ; 639/925/927/351 ; Bacteriophage lambda - chemistry ; Biosensing Techniques ; Compression ; Deoxyribonucleic acid ; DNA ; DNA - chemistry ; DNA - ultrastructure ; DNA sequencing ; Elastomers ; Humanities and Social Sciences ; multidisciplinary ; Nanopores - ultrastructure ; Nanotechnology - instrumentation ; Nanotechnology - methods ; Polymers ; Science ; Sequence Analysis, DNA - methods</subject><ispartof>Scientific reports, 2012-11, Vol.2 (1), p.791, Article 791</ispartof><rights>The Author(s) 2012</rights><rights>Copyright Nature Publishing Group Nov 2012</rights><rights>Copyright © 2012, Macmillan Publishers Limited. All rights reserved 2012 Macmillan Publishers Limited. All rights reserved</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593</citedby><cites>FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/1897401492/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/1897401492?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,44589,53790,53792,74897</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23145315$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fanzio, Paola</creatorcontrib><creatorcontrib>Manneschi, Chiara</creatorcontrib><creatorcontrib>Angeli, Elena</creatorcontrib><creatorcontrib>Mussi, Valentina</creatorcontrib><creatorcontrib>Firpo, Giuseppe</creatorcontrib><creatorcontrib>Ceseracciu, Luca</creatorcontrib><creatorcontrib>Repetto, Luca</creatorcontrib><creatorcontrib>Valbusa, Ugo</creatorcontrib><title>Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device</title><title>Scientific reports</title><addtitle>Sci Rep</addtitle><addtitle>Sci Rep</addtitle><description>Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation and is promising for further development in sequencing technology.</description><subject>631/57</subject><subject>639/766/25</subject><subject>639/925/350</subject><subject>639/925/927/351</subject><subject>Bacteriophage lambda - chemistry</subject><subject>Biosensing Techniques</subject><subject>Compression</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA - chemistry</subject><subject>DNA - ultrastructure</subject><subject>DNA sequencing</subject><subject>Elastomers</subject><subject>Humanities and Social Sciences</subject><subject>multidisciplinary</subject><subject>Nanopores - ultrastructure</subject><subject>Nanotechnology - instrumentation</subject><subject>Nanotechnology - methods</subject><subject>Polymers</subject><subject>Science</subject><subject>Sequence Analysis, DNA - methods</subject><issn>2045-2322</issn><issn>2045-2322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><recordid>eNplkUtLAzEUhYMottQu_AMy4EphNK95ZCOU1hfYKtiuQybJtFOmSU06hf57I1NLxWSRkPNx7sm9AFwieIcgye-902sIM4ZOQBdDmsSYYHx6dO-AvvdLGFaCGUXsHHQwQTQhKOmC2diqphabysyj0WQQTZ0wvrYyvFgTFbtIRENrNs7WtVbRSJfWrVrNlkH7GI0_o4kwVi6EMboOxLaS-gKclaL2ur8_e2D29DgdvsRv78-vw8FbLBNIN3EhoJJKScKUwKnEaa5KiSDOZFkwmRZYZanOoCrC78LOcp3LQmeIKpYLnTDSAw-t77opVlpJHZKKmq9dtRJux62o-F_FVAs-t1tOKKMkRcHgem_g7Fej_YYvbeNMyMxRzjIKEWU4UDctJZ31od_loQKC_GcI_DCEwF4dRzqQvy0PwG0L-CCZuXZHJf-5fQMoKZFy</recordid><startdate>20121109</startdate><enddate>20121109</enddate><creator>Fanzio, Paola</creator><creator>Manneschi, Chiara</creator><creator>Angeli, Elena</creator><creator>Mussi, Valentina</creator><creator>Firpo, Giuseppe</creator><creator>Ceseracciu, Luca</creator><creator>Repetto, Luca</creator><creator>Valbusa, Ugo</creator><general>Nature Publishing Group UK</general><general>Nature Publishing Group</general><scope>C6C</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>5PM</scope></search><sort><creationdate>20121109</creationdate><title>Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device</title><author>Fanzio, Paola ; Manneschi, Chiara ; Angeli, Elena ; Mussi, Valentina ; Firpo, Giuseppe ; Ceseracciu, Luca ; Repetto, Luca ; Valbusa, Ugo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>631/57</topic><topic>639/766/25</topic><topic>639/925/350</topic><topic>639/925/927/351</topic><topic>Bacteriophage lambda - chemistry</topic><topic>Biosensing Techniques</topic><topic>Compression</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA - chemistry</topic><topic>DNA - ultrastructure</topic><topic>DNA sequencing</topic><topic>Elastomers</topic><topic>Humanities and Social Sciences</topic><topic>multidisciplinary</topic><topic>Nanopores - ultrastructure</topic><topic>Nanotechnology - instrumentation</topic><topic>Nanotechnology - methods</topic><topic>Polymers</topic><topic>Science</topic><topic>Sequence Analysis, DNA - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fanzio, Paola</creatorcontrib><creatorcontrib>Manneschi, Chiara</creatorcontrib><creatorcontrib>Angeli, Elena</creatorcontrib><creatorcontrib>Mussi, Valentina</creatorcontrib><creatorcontrib>Firpo, Giuseppe</creatorcontrib><creatorcontrib>Ceseracciu, Luca</creatorcontrib><creatorcontrib>Repetto, Luca</creatorcontrib><creatorcontrib>Valbusa, Ugo</creatorcontrib><collection>SpringerOpen (Open Access)</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Biological Sciences</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Scientific reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fanzio, Paola</au><au>Manneschi, Chiara</au><au>Angeli, Elena</au><au>Mussi, Valentina</au><au>Firpo, Giuseppe</au><au>Ceseracciu, Luca</au><au>Repetto, Luca</au><au>Valbusa, Ugo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device</atitle><jtitle>Scientific reports</jtitle><stitle>Sci Rep</stitle><addtitle>Sci Rep</addtitle><date>2012-11-09</date><risdate>2012</risdate><volume>2</volume><issue>1</issue><spage>791</spage><pages>791-</pages><artnum>791</artnum><issn>2045-2322</issn><eissn>2045-2322</eissn><abstract>Several strategies have been developed for the control of DNA translocation in nanopores and nanochannels. However, the possibility to reduce the molecule speed is still challenging for applications in the field of single molecule analysis, such as ultra-rapid sequencing. This paper demonstrates the possibility to alter the DNA translocation process through an elastomeric nanochannel device by dynamically changing its cross section. More in detail, nanochannel deformation is induced by a macroscopic mechanical compression of the polymeric device. This nanochannel squeezing allows slowing down the DNA molecule passage inside it. This simple and low cost method is based on the exploitation of the elastomeric nature of the device, can be coupled with different sensing techniques, is applicable in many research fields, such as DNA detection and manipulation and is promising for further development in sequencing technology.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>23145315</pmid><doi>10.1038/srep00791</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 2045-2322
ispartof Scientific reports, 2012-11, Vol.2 (1), p.791, Article 791
issn 2045-2322
2045-2322
language eng
recordid cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3494361
source Publicly Available Content Database; Full-Text Journals in Chemistry (Open access); PubMed Central; Springer Nature - nature.com Journals - Fully Open Access
subjects 631/57
639/766/25
639/925/350
639/925/927/351
Bacteriophage lambda - chemistry
Biosensing Techniques
Compression
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA - ultrastructure
DNA sequencing
Elastomers
Humanities and Social Sciences
multidisciplinary
Nanopores - ultrastructure
Nanotechnology - instrumentation
Nanotechnology - methods
Polymers
Science
Sequence Analysis, DNA - methods
title Modulating DNA Translocation by a Controlled Deformation of a PDMS Nanochannel Device
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T23%3A47%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Modulating%20DNA%20Translocation%20by%20a%20Controlled%20Deformation%20of%20a%20PDMS%20Nanochannel%20Device&rft.jtitle=Scientific%20reports&rft.au=Fanzio,%20Paola&rft.date=2012-11-09&rft.volume=2&rft.issue=1&rft.spage=791&rft.pages=791-&rft.artnum=791&rft.issn=2045-2322&rft.eissn=2045-2322&rft_id=info:doi/10.1038/srep00791&rft_dat=%3Cproquest_pubme%3E1897401492%3C/proquest_pubme%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c504t-ba0dcddc39da26c268dfc1027cfb9c6b2d76e70db79191978e8cbe714d98ae593%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1897401492&rft_id=info:pmid/23145315&rfr_iscdi=true