Correlative light and electron microscopy using cathodoluminescence from nanoparticles with distinguishable colours

Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images and rapid optical signal degradation under electron beam irradi...

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Bibliographic Details
Published in:Scientific reports 2012-11, Vol.2 (1), p.865-865, Article 865
Main Authors: Glenn, D. R., Zhang, H., Kasthuri, N., Schalek, R., Lo, P. K., Trifonov, A. S., Park, H., Lichtman, J. W., Walsworth, R. L.
Format: Article
Language:English
Subjects:
639/301/930/328/1649
639/624
639/638/925/930/328
639/925/357/354
Electron microscopes
Electron microscopy
Humanities and Social Sciences
Mapping
multidisciplinary
Nanoparticles
Quantum dots
Science
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Summary:Correlative light and electron microscopy promises to combine molecular specificity with nanoscale imaging resolution. However, there are substantial technical challenges including reliable co-registration of optical and electron images and rapid optical signal degradation under electron beam irradiation. Here, we introduce a new approach to solve these problems: imaging of stable optical cathodoluminescence emitted in a scanning electron microscope by nanoparticles with controllable surface chemistry. We demonstrate well-correlated cathodoluminescence and secondary electron images using three species of semiconductor nanoparticles that contain defects providing stable, spectrally-distinguishable cathodoluminescence. We also demonstrate reliable surface functionalization of the particles. The results pave the way for the use of such nanoparticles for targeted labeling of surfaces to provide nanoscale mapping of molecular composition, indicated by cathodoluminescence colour, simultaneously acquired with structural electron images in a single instrument.
ISSN:2045-2322
2045-2322
DOI:10.1038/srep00865