DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA

MicroRNAs (miRNAs) have been identified as promising cancer biomarkers due to their stable presence in serum. As an alternative to PCR-based homogenous assays, surface-based electrochemical biosensors offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. N...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2012-11, Vol.2 (1), p.867, Article 867
Main Authors: Wen, Yanli, Pei, Hao, Shen, Ye, Xi, Junjie, Lin, Meihua, Lu, Na, Shen, Xizhong, Li, Jiong, Fan, Chunhai
Format: Article
Language:English
Subjects:
631/337/384/521
639/301/1005/1009
639/638/161
639/925/350
Biomarkers
Biosensing Techniques
Biosensors
Cancer
Carcinoma, Squamous Cell - genetics
Carcinoma, Squamous Cell - metabolism
Carcinoma, Squamous Cell - pathology
Crowding
Deoxyribonucleic acid
DNA
DNA - chemistry
Electrochemical Techniques
Electrochemistry
Electrodes
Engineering
Enzymes
Esophageal Neoplasms - genetics
Esophageal Neoplasms - metabolism
Esophageal Neoplasms - pathology
Esophagus
Gold - chemistry
Humanities and Social Sciences
Humans
Mass transport
MicroRNAs
MicroRNAs - analysis
miRNA
multidisciplinary
Nanostructures - chemistry
Point-of-Care Systems
Polymerase Chain Reaction
Science
Sensors
Signal transduction
Squamous cell carcinoma
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) have been identified as promising cancer biomarkers due to their stable presence in serum. As an alternative to PCR-based homogenous assays, surface-based electrochemical biosensors offer great opportunities for low-cost, point-of-care tests (POCTs) of disease-associated miRNAs. Nevertheless, the sensitivity of miRNA sensors is often limited by mass transport and crowding effects at the water-electrode interface. To address such challenges, we herein report a DNA nanostructure-based interfacial engineering approach to enhance binding recognition at the gold electrode surface and drastically improve the detection sensitivity. By employing this novel strategy, we can directly detect as few as attomolar (
ISSN:2045-2322
2045-2322
DOI:10.1038/srep00867