Dietary supplementation of some antioxidants against hypoxia

• Published in: World journal of gastroenterology : WJG 2012-11, Vol.18 (44), p.6379-6386
• Main Authors: Ali, Sanaa Ahmed, Aly, Hanan Farouk, Faddah, Lilla Mohammed, Zaidi, Zeenat F
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - pharmacology; Arginine - pharmacology; Chemical and Drug Induced Liver Injury - etiology; Chemical and Drug Induced Liver Injury - metabolism; Chemical and Drug Induced Liver Injury - pathology; Chemical and Drug Induced Liver Injury - prevention & control; Cytoprotection; Dietary Supplements; Disease Models, Animal; Drug Therapy, Combination; Energy Metabolism - drug effects; Hypoxia - chemically induced; Hypoxia - metabolism; Hypoxia - pathology; Hypoxia - prevention & control; Liver - drug effects; Liver - metabolism; Liver - pathology; Male; Melatonin - pharmacology; Oxidative Stress - drug effects; Rats; Reactive Oxygen Species - metabolism; Sodium Nitrite; Time Factors; Topic Highlight; Ubiquinone - analogs & derivatives; Ubiquinone - pharmacology; 三磷酸腺苷; 亚硝酸钠; 抗氧化剂; 碱性磷酸酶; 糖原磷酸化酶; 组织病理学检查; 缺氧保护; 谷丙转氨酶
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i44.6379

The present study aims to clarify the protective effect of supplementation with some antioxidants, such as ide- benone （200 mg/kg, ip）, melatonin （10 mg/kg, ip） and arginine （200 mg/kg, ip） and their combination, on liver function （T. protein, albumin, alanine aminotransferase, aspartate aminotransferase and alkaline phosphatase）, energetic parameters （adenosine triphosphate, adenos- ine diphosphate, adenosine monophosphate, inorganic phosphate, total adenylate, adenylate energy charge and potential phosphate）. The effect on glycolytic and glycogenolytic enzymes （glucose, glycogen, glycogen phosphorylase, pyruvate kinase and phosphofructoki- nase against hypoxia） was also studied. The drugs were administered 24 and 1 h prior sodium nitrite intoxica- tion. All biochemical parameters were estimated 1 h after sodium nitrite injection. Injection of sodium nitrite （75 mg/kg, sc） produced a significant disturbance inall biochemical parameters of liver function, energetic parameters and glycolytic and glycogenolytic enzymes. Hepatic damage was confirmed by histopathological examination of the liver as compared to controls. The marked changes in hepatic cells induced by sodium nitrite were completely abolished by pretreatment with the drug combination, suggesting potential protection against sodium nitrite-induced hypoxia. It could be concluded that a combination of both idebenone and melatonin or idebenone and arginine provides potential protection against sodium nitrite-induced hypoxia by improving biochemical parameters and preserving liver histology.