Optimizing cryoprotectant perfusion conditions for intact ovary: a bovine model

Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusio...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 2012-11, Vol.29 (11), p.1255-1260
Main Authors: Zhang, Jian-Min, Zhang, Ying-Chun, Ruan, Li-Hong, Wang, Heng-Cai
Format: Article
Language:English
Subjects:
Animals
Cattle
Cryopreservation
Cryopreservation - methods
Cryoprotective Agents
Female
Fertility Preservation
Follicles
Gynecology
Human Genetics
Humans
Medicine
Medicine & Public Health
Models, Animal
Organ Preservation - methods
Ovarian Follicle - physiology
Ovarian Follicle - ultrastructure
Ovaries
Ovary - cytology
Ovary - physiology
Perfusion - instrumentation
Perfusion - methods
Reproductive Medicine
Veins & arteries
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Summary:Purpose The aim of this study was to detect the effects of different perfusion pressure and different length of perfusion period on whole ovarian cryopreservation Methods Bovine whole ovaries were vitrified-warmed. The ovaries were divided into the experimental groups according to different perfusion pressure and different length of perfusion period. Follicular viability was assessed using the trypan blue test; the percentage of morphologically normal primordial follicles and the 17-β estradiol level in the culture supernatants were measured. Results When perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min, the viability of ovarian tissues in bovine whole ovarian cryopreservation were higher than other protocols. Conclusion Protocol IIb (the perfusion pressure was 100 mmHg, and the length of perfusion period was 40 min) was appropriate for bovine whole ovarian cryopreservation.
ISSN:1058-0468
1573-7330
DOI:10.1007/s10815-012-9845-4