A Novel Herbal Medicine KIOM-MA Exerts an Anti-Inflammatory Effect in LPS-Stimulated RAW 264.7 Macrophage Cells

KIOM-MA was recently reported as a novel herbal medicine effective for atopic dermatitis and asthma. In this study, we have demonstrated the inhibitory effect of KIOM-MA on proinflammatory mediator produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. KIOM-MA significantly inhibited the e...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-11
Main Authors: Ma, Jin Yeul, Song, Kwang Hoon, Hwang, Youn-Hwan, Kim, Aeyung, Im, Ga Young, Jeong, Yun Hee, Cho, Won-Kyung, Oh, You-Chang, Kim, Taesoo
Format: Article
Language:English
Subjects:
Anti-inflammatory agents
Archives & records
Asthma
Atopic dermatitis
Cyclooxygenase-2
Cytokines
Dermatitis
Eczema
Extracellular signal-regulated kinase
Gelatinase
Gelatinase B
Gene expression
Herbal medicine
Inflammation
Inflammatory diseases
Interleukin 6
JNK protein
Kinases
Leukocyte migration
Lipopolysaccharides
Macrophages
Matrix metalloproteinase
Medicine
Metalloproteinase
NF-κB protein
Nitric oxide
Nitric-oxide synthase
Pharmacology
Prostaglandin E2
Rodents
Transcription factors
Tumor necrosis factor-α
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Summary:KIOM-MA was recently reported as a novel herbal medicine effective for atopic dermatitis and asthma. In this study, we have demonstrated the inhibitory effect of KIOM-MA on proinflammatory mediator produced in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. KIOM-MA significantly inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) as well as nitric oxide (NO) and prostaglandin E2 (PGE2). Consistent with the inhibitory effect on PGE2, KIOM-MA suppresses the LPS-induced migration of macrophages and gelatinase activity and the expression of matrix metalloprotease-9 (MMP-9) in a dose-dependent manner. Additionally, KIOM-MA showed a strong suppressive effect on the inflammatory cytokines production such as tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). We also found that KIOM-MA inhibits the activation of nuclear factor-κB (NF-κB) and represses the activity of extracellular signal-regulated kinase (ERK), p38, and c-Jun NH2-terminal kinase (JNK) mitogen-activated protein kinases (MAPKs). Taken together, we elucidated the mechanism of anti-inflammatory effect of KIOM-MA using RAW 264.7 cells stimulated by LPS.
ISSN:1741-427X
1741-4288
1741-4288
DOI:10.1155/2012/462383