Epimedium koreanum Nakai Water Extract Exhibits Antiviral Activity against Porcine Epidermic Diarrhea Virus In Vitro and In Vivo

Porcine epidemic diarrhea virus (PEDV) causes diarrhea of pigs age-independently and death of young piglets, resulting in economic loss of porcine industry. We have screened 333 natural oriental herbal medicines to search for new antiviral candidates against PEDV. We found that two herbal extracts,...

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Bibliographic Details
Published in:Evidence-based complementary and alternative medicine 2012-01, Vol.2012 (2012), p.1-10
Main Authors: Yang, Hye Jin, Yim, Nam-Hui, Choi, Yu Jeong, Kim, Hyunil, Cho, Won-Kyung, Ma, Jin Yeul
Format: Article
Language:English
Subjects:
Age
Angiogenesis
Antiviral activity
Antiviral drugs
Apoptosis
Biopsy
Cancer therapies
Cytomegalovirus
Cytotoxicity
Diarrhea
Epidemics
Epimedium koreanum
Flavonoids
Gene expression
Hepatitis
Herbal medicine
Herpes viruses
HIV
Human immunodeficiency virus
Infections
Influenza
Intestine
Lonicera japonica
Oral administration
Prostate cancer
Respiratory syncytial virus
Swine
Transmissible gastroenteritis
Viral infections
Virology
Viruses
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Summary:Porcine epidemic diarrhea virus (PEDV) causes diarrhea of pigs age-independently and death of young piglets, resulting in economic loss of porcine industry. We have screened 333 natural oriental herbal medicines to search for new antiviral candidates against PEDV. We found that two herbal extracts, KIOM 198 and KIOM 124, contain significant anti-PED viral effect. KIOM 198 and KIOM 124 were identified as Epimedium koreanum Nakai and Lonicera japonica Thunberg, respectively. The further plaque and CPE inhibition assay in vitro showed that KIOM 198 has much stronger antiviral activity than KIOM 124. Additionally, KIOM 198 exhibited a similar extent of antiviral effect against other subtypes of Corona virus such as sm98 and TGE viruses. Cytotoxicity results showed that KIOM 198 is nontoxic on the cells and suggest that it can be delivered safely for therapy. Furthermore, when we orally administered KIOM 198 to piglets and then infected them with PEDV, the piglets did not show any disease symptoms like diarrhea and biopsy results showed clean intestine, whereas control pigs without KIOM 198 treatment exhibited PED-related severe symptoms. These results imply that KIOM 198 contains strong antiviral activity and has a potential to be developed as an antiviral phytomedicine to treat PEDV-related diseases in pigs.
ISSN:1741-427X
1741-4288
DOI:10.1155/2012/985151