Histo-anatomical structure of the living isolated rat heart in two contraction states assessed by diffusion tensor MRI

Deformation and wall-thickening of ventricular myocardium are essential for cardiac pump function. However, insight into the histo-anatomical basis for cardiac tissue re-arrangement during contraction is limited. In this report, we describe dynamic changes in regionally prevailing cardiomyocyte (fib...

Full description

Saved in:
Bibliographic Details
Published in:Progress in biophysics and molecular biology 2012-10, Vol.110 (2-3), p.319-330
Main Authors: Hales, Patrick W., Schneider, Jürgen E., Burton, Rebecca A.B., Wright, Benjamin J., Bollensdorff, Christian, Kohl, Peter
Format: Article
Language:English
Subjects:
Animals
Cardiac contraction
Cardiac magnetic resonance imaging
Diffusion Tensor Imaging
Female
Heart - anatomy & histology
Muscle Contraction
Myocardial histo-architecture
Myocardium - cytology
Myolaminae
Original Research
Perfusion
Rats
Rats, Sprague-Dawley
Tissue Survival
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Deformation and wall-thickening of ventricular myocardium are essential for cardiac pump function. However, insight into the histo-anatomical basis for cardiac tissue re-arrangement during contraction is limited. In this report, we describe dynamic changes in regionally prevailing cardiomyocyte (fibre) and myolaminar (sheet) orientations, using Diffusion Tensor Imaging (DTI) of ventricles in the same living heart in two different mechanical states. Hearts, isolated from Sprague–Dawley rats, were Langendorff-perfused and imaged, initially in their slack state during cardioplegic arrest, then during lithium-induced contracture. Regional fibre- and sheet-orientations were derived from DTI-data on a voxel-wise basis. Contraction was accompanied with a decrease in left-handed helical fibres (handedness relative to the baso-apical direction) in basal, equatorial, and apical sub-epicardium (by 14.0%, 17.3%, 15.8% respectively; p 
ISSN:0079-6107
1873-1732
DOI:10.1016/j.pbiomolbio.2012.07.014