Decontamination of dental implant surfaces by means of photodynamic therapy

• Published in: Lasers in medical science 2013-01, Vol.28 (1), p.303-309
• Main Authors: Marotti, Juliana, Tortamano, Pedro, Cai, Silvana, Ribeiro, Martha Simões, Franco, João Eduardo Miranda, de Campos, Tomie Toyota
• Format: Article
• Language: English
• Subjects: Antimicrobial agents; Decontamination - methods; Dental Implants - microbiology; Dental research; Dentistry; Humans; In Vitro Techniques; Lasers; Medicine; Medicine & Public Health; Methylene Blue; Optical Devices; Optics; Original; Original Article; Peri-Implantitis - drug therapy; Peri-Implantitis - microbiology; Photochemotherapy - methods; Photodynamic therapy; Photonics; Quantum Optics; Statistics, Nonparametric; Surface Properties; Titanium; Transplants & implants
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-012-1148-6

Several implant surface debridement methods have been reported for the treatment of peri-implantitis, however, some of them can damage the implant surface or promote bacterial resistance. Photodynamic therapy (PDT) is a new treatment option for peri-implantitis. The aim of this in vitro study was to analyze implant surface decontamination by means of PDT. Sixty implants were equally distributed ( n  = 10) into four groups and two subgroups. In group G1 there was no decontamination, while in G2 decontamination was performed with chlorhexidine. G3 (PDT − laser + dye) and G4 (laser, without dye) were divided into two subgroups each; with PDT performed for 3 min in G3a and G4a, and for 5 min in G3b and G4b. After 5 min in contact with methylene blue dye (G3), the implants were irradiated (G3 and G4) with a low-level laser (GaAlAs, 660 nm, 30 mW) for 3 or 5 min (7.2 and 12 J). After the dilutions, culture media were kept in an anaerobic atmosphere for 1 week, and then colony forming units were counted. There was a significant difference ( p