Single-molecule imaging in live bacteria cells

Bacteria, such as Escherichia coli and Caulobacter crescentus, are the most studied and perhaps best-understood organisms in biology. The advances in understanding of living systems gained from these organisms are immense. Application of single-molecule techniques in bacteria have presented unique d...

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Bibliographic Details
Published in:Philosophical transactions of the Royal Society of London. Series B. Biological sciences 2013-02, Vol.368 (1611), p.20120355-20120355
Main Authors: Ritchie, Ken, Lill, Yoriko, Sood, Chetan, Lee, Hochan, Zhang, Shunyuan
Format: Article
Language:English
Subjects:
Bacteria
Bacterial Outer Membrane Proteins - chemistry
Biophysics
Caulobacter crescentus
Cell Membrane - chemistry
Cytoplasm - chemistry
Diffusion
Escherichia coli
Escherichia coli - chemistry
Escherichia coli - genetics
Escherichia coli Proteins - chemistry
Fluorescent Dyes - chemistry
Gene Expression Regulation, Bacterial
Imaging
Membrane Proteins - chemistry
Membrane Transport Proteins - chemistry
Molecular Imaging - methods
Nanotechnology
Protein Transport
Review
Sensitivity and Specificity
Single Molecule
Staining and Labeling
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Summary:Bacteria, such as Escherichia coli and Caulobacter crescentus, are the most studied and perhaps best-understood organisms in biology. The advances in understanding of living systems gained from these organisms are immense. Application of single-molecule techniques in bacteria have presented unique difficulties owing to their small size and highly curved form. The aim of this review is to show advances made in single-molecule imaging in bacteria over the past 10 years, and to look to the future where the combination of implementing such high-precision techniques in well-characterized and controllable model systems such as E. coli could lead to a greater understanding of fundamental biological questions inaccessible through classic ensemble methods.
ISSN:0962-8436
1471-2970
DOI:10.1098/rstb.2012.0355